A modular single-cell pipette microfluidic chip coupling to ETAAS and ICP-MS for single cell analysis  

作　　者：Xing Wei Meng Yang Ze Jiang Jinhui Liu Xuan Zhang Mingli Chen Jianhua Wang 

机构地区：[1]Research Center for Analytical Sciences,Department of Chemistry,College of Sciences,Northeastern University,Shenyang 110819,China

出　　处：《Chinese Chemical Letters》2022年第3期1373-1376,共4页中国化学快报（英文版）

基　　金：financial support of the National Natural Science Foundation of China (Nos. 21727811,21922402);the Fundamental Research Funds for the Central Universities (Nos. N2005003, N2105017);the Liaoning Revitalization Talents Program (No. XLYC1802016);Scientific Research Funding Project of the Education Department of Liaoning (No. LJKZ0007)。

摘　　要：Accurate single-cell capture is a crucial step for single cell biological and chemical analysis. Conventional single-cell capturing often confront operational complexity, limited efficiency, cell damage, large scale but low accuracy, incompetence in the acquirement of nano-upgraded single-cell liquid. Flow cytometry has been widely used in large-scale single-cell detection, while precise single-cell isolation relies on both a precision operating platform and a microscope, which is not only extremely inefficient, but also not conducive to couple with modern analytical instruments. Herein, we develop a modular single-cell pipette(m SCP) microfluidic chip with high efficiency and strong applicability for accurate direct capture of single viable cell from cell suspensions into nanoliter droplets(30-1000 n L). The m SCP is used as a sampling platform for the detection of Cd Te quantum dots in single cells with electrothermal atomic absorption spectrometry(ETAAS) for the first time. It also ensures precise single-cell sampling and detection by inductively coupled plasma mass spectrometry(ICP-MS).

关 键 词：Single-cell pipette Microfluidic chip Single-cell capture ETAAS ICP-MS CdTe QDs 

分 类 号：Q2-33[生物学—细胞生物学] O652[理学—分析化学]