Programmed co-assembly of DNA-peptide hybrid microdroplets by phase separation  

作　　者：Shengtao Yao Yue Liao Rizhao Pan Weiping Zhu Yufang Xu Yangyang Yang Xuhong Qian 

机构地区：[1]School of Pharmacy,Shanghai Key Laboratory of Chemical Biology,East China University of Science and Technology,Shanghai 200237,China [2]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology Shanghai 200237,China

出　　处：《Chinese Chemical Letters》2022年第3期1545-1549,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 31600802)。

摘　　要：Biopolymers, including DNA and peptides have been used as excellent self-assembling building blocks for programmable single-component or hybrid materials, due to their controlled molecular interactions.However, combining two assembling principles of DNA-based programmability and peptide-based specific molecular interactions for hybrid structures to microscale has not yet been achieved. In this study,we describe a hybrid microsystem that emerges from the co-assembly of DNA origami structure and short elastin-like polypeptide conjugated oligonucleotides, and initiates liquid-liquid phase separation to generate microdroplets upon heating above the transition temperature. Moreover, the hybrid microdroplets are capable for guest molecule trapping and perform bi-/tri-enzymatic cascades with rate enhancements as open “microreactors”. Our programmed assembled DNA-peptide microsystem represents a new combination of DNA nanotechnology and peptide science and opens potential application routes toward lifeinspired biomaterials.

关 键 词：SELF-ASSEMBLY MICRODROPLET DNA origami Phase separation Elastin-like polypeptides 

分 类 号：Q503[生物学—生物化学] TB30[一般工业技术—材料科学与工程]