A chemical labeling of N^(6)-formyl adenosine(f^(6)A) RNA  

作　　者：Li-Jun Xie Cui-Lian Lin Li Liu Liang Cheng 

机构地区：[1]Beijing National Laboratory for Molecular Sciences(BNLMS),CAS Key Laboratory of Molecular Recognition and Function,CAS Research/Education Center for Excellence in Molecular Sciences,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China [2]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Chinese Chemical Letters》2022年第3期1563-1566,共4页中国化学快报（英文版）

基　　金：supported by the National Key R&D Program of China (Nos. 2017YFA0208100 and 2020YFA0707901);National Natural Science Foundation of China (Nos. 22022704, 91853124,21977097 and 21778057);Postdoctoral Innovative Talents Support Program (No. BX20200337, to Dr. L.-J. Xie);Chinese Academy of Sciences。

摘　　要：N^(6)-methyl adenosine (m^(6)A) is an eminent epigenetic mark in mRNAs that affects a broad range of biological functions in diverse species. However, the chemically inert methyl group prevents a direct labeling of this modification for subsequent detection and sequencing. Therefore, most current approaches for the labeling of m^(6)A still have limitations of relying on the utilization of corresponding methyltransferases, which resulted in the lacking of efficiency. Here we present an approach which selectively alkylated the N^(6)-formyl adenosine (f^(6)A), the key intermediate during chemical oxidation of m^(6)A, with an alkyne functionality that can be further labeled with click reactions. This covalent labeling approach will be able to facilitate in the affinity purification, detection and genome-wide profiling studies.

关 键 词：RNA epigenetics Chemical modulation LABELING Nucleic acids ALKYLATION 

分 类 号：Q522[生物学—生物化学]