低氧-复氧胁迫对鲢抗氧化酶活性及Cu/Zn-SOD和Mn-SOD基因表达的影响  

HYPOXIA-REOXYGENATION STRESS ON ANTIOXIDANT ENZYME ACTIVITY AND EXPRESSION OF CU/ZN-SOD AND MN-SOD GENES IN SILVER CARP(HYPOPHTHALMICHTHYS MOLITRIX)

作　　者：张美东 凌晨 沙航 陈梦 王丹[1] 罗相忠[1] 邹桂伟[1] 梁宏伟[1] ZHANG Mei-Dong;LING Chen;SHA Hang;CHEN Meng;WANG Dan;LUO Xiang-Zhong;ZOU Gui-Wei;LIANG Hong-Wei(Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China;College of Fisheries,Huazhong Agricultural University,Wuhan 430000,China)

机构地区：[1]中国水产科学研究院长江水产研究所,武汉430223 [2]华中农业大学水产学院,武汉430000

出　　处：《水生生物学报》2022年第4期498-506,共9页Acta Hydrobiologica Sinica

基　　金：国家重点研发计划(2018YFD0900302);国家大宗淡水鱼产业技术体系(CARS-45);农业农村部淡水渔业健康养殖重点实验室(ZJK202109)资助。

摘　　要：为探究低氧-复氧胁迫对鲢(Hypophthalmichthys molitrix)抗氧化酶活性及Cu/Zn-SOD和Mn-SOD基因表达的影响,对鲢进行急性低氧、持续低氧及复氧实验,进而分析血清、心脏和肝脏中不同抗氧化酶和SODs基因表达的变化特征。结果表明:在急性低氧胁迫后,血清中总抗氧化能力(T-AOC)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-PX)活性随着氧浓度的降低均呈上升趋势,但超氧化物歧化酶(SOD)活性呈先升后降的趋势。在持续低氧胁迫后,血清中T-AOC和GSH-PX活性随着低氧胁迫时间的增加显著升高(P<0.05);心脏中SOD活性显著高于常氧水平(P<0.05),但Cu/Zn-SOD和Mn-SOD基因表达在低氧胁迫24h时显著低于常氧水平(P<0.05);肝脏中SOD活性在低氧胁迫24h时显著高于常氧水平(P<0.05),且Cu/Zn-SOD和Mn-SOD基因表达在低氧胁迫24h时也显著高于常氧水平(P<0.05)。复氧后,血清、心脏和肝脏中T-AOC、SOD、CAT和GSHPX活性均能恢复至常氧水平,且心脏和肝脏中Cu/Zn-SOD和Mn-SOD基因表达的也能恢复至常氧水平,但肝脏中Mn-SOD基因表达恢复至常氧水平较在心脏中所需时间更少。因而,鲢可以通过调节抗氧化酶的活性来保护自身免受氧化应激造成的损伤。研究为解析低氧胁迫下鲢抗氧化应激机制提供了基础。Hypophthalmichthys molitrix accounts for an important proportion in Chinese aquaculture and is easily affected by the hypoxia stress.To explore the effect of hypoxia-reoxygenation stress on antioxidant enzyme activity and mRNA expression levels of Cu/Zn-SOD and Mn-SOD genes,H.molitrix with the body weight of(100±10)g was used to hypoxia experimental.Nitrogen and oxygen were inputted into the water to maintain the oxygen concentration at 2.5,1.5,0.5 and 0.25 mg/L for 0.5h under hypoxia stress,and at oxygen concentration of 2.5 mg/L for 3h,6h,12h and 24h,moreover,reoxygenation(dissolved oxygen 6.5±0.3 mg/L)for 3h,6h,12h and 24h in 2.5 mg/L after 24h,enzyme activity and gene expression of serum,heart and liver tissue was analyzed.The results were as follows:with the decrease of oxygen concentration the activities of total antioxidant capacity(T-AOC),catalase(CAT)and glutathione peroxidase(GSH-PX)in serum were increased,but the activity of superoxide dismutase(SOD)was firstly increased and then decreased,which was slightly lower than the normoxic level at the dissolved oxygen concentration of 0.25 mg/L.However,the SOD activity of heart and liver tissues was significantly higher than that of the normoxic level(P<0.05).The expression level of Cu/Zn-SOD and Mn-SOD of heart and liver also increased with the decrease of oxygen concentration,and the expression of Cu/Zn-SOD and Mn-SOD was significantly higher than that of the normoxic oxygen at the dissolved oxygen concentration of 2.5 mg/L(P<0.05).With the increase of hypoxia time,the activities of T-AOC,CAT and GSH-PX in serum increased significantly(P<0.05),and were significantly higher than the normoxic level after 6h hypoxia stress(P<0.05).The CAT activity in heart was lower than the normoxic level during hypoxic stress,while the SOD activity was significantly higher than the normoxic level(P<0.05),but the Cu/Zn-SOD and Mn-SOD genes were significantly lower than the normoxic level at 24h(P<0.05).The activities of T-AOC,SOD and GSH-PX of liver were significantly lower

关 键 词：低氧胁迫 抗氧化酶 Cu/Zn-SOD基因 Mn-SOD基因 鲢 

分 类 号：S965.1[农业科学—水产养殖]