蒲公英水提液不同萃取部位对人骨肉瘤MG-63细胞的影响  

Effects of Different Parts of Dandelion Water Extract on Human Osteosarcoma MG-63 Cells

作　　者：徐世红[1,2] 柳博 谢兴文 李鼎鹏 许伟[2] 丁聚贤 黄睿 姜朝阳 XU Shihong;LIU Bo;XIE Xingwen;LI Dingpeng;XU Wei;DING Juxian;HUANG Rui;JIANG Chaoyang(Gansu Provincial Academy of Chinese Medicine,Lanzhou 730050,China;Gansu Provincial Hospital of Traditional Chinese Medicine;Gansu University of Chinese Medicine;Second Provincial People's Hospital of Gansu)

机构地区：[1]甘肃省中医药研究院,甘肃兰州730050 [2]甘肃省中医院 [3]甘肃中医药大学 [4]甘肃省第二人民医院

出　　处：《西部中医药》2022年第5期21-24,共4页Western Journal of Traditional Chinese Medicine

基　　金：甘肃省青年科技基金(18JR3RA068)。

摘　　要：目的:探讨蒲公英水提液不同萃取部位对人骨肉瘤MG-63细胞的细胞形态及增殖的影响。方法:将蒲公英加水煎煮得蒲公英水提液,然后用石油醚、乙酸乙酯和正丁醇依次以1∶1比例萃取蒲公英水提物,得到不同溶剂萃取部位;人骨肉瘤MG-63细胞传代贴壁培养后应用蒲公英水提液不同萃取部位培养;采用四甲基偶氮唑盐(methyl thiazolyl tetrazdium,MTT)法检测蒲公英水提液各萃取部位在不同时间内对人骨肉瘤细胞MG-63的增殖抑制作用。结果:蒲公英水提液各萃取部位在体外对MG-63细胞增殖具有抑制作用,呈现出剂量与时间的效应关系。结论:蒲公英水提液各萃取部位能抑制人骨肉瘤MG-63细胞,在体外有一定抗肿瘤活性,其中以乙酸乙酯萃取部位最有效。Objective:To explore the effects of different parts of dandelion water extract on the cellular morphology and proliferation of human osteosarcoma(OS) MG-63 cells.Methods:Water extract of dandelion was obtained by decocting the whole dandelion with water;different solvent extracts were got by extracting dandelion water extract with petroleum ether,ethyl acetate and n-butanol in the ratio of 1:1,different extracts of dandelion were applied to culture human osteosarcoma MG-63 cells after passage and adherent culture;MTT method was adopted to detect the inhibitory effects of dandelion water extract on the proliferation of human osteosarcoma MG-63cells at different times.Results:Dandelion water extract could inhibit the proliferation of MG-63 cells in vitro,presenting in the dose-time concentration-effect relationship.Conclusion:Dandelion water extract could inhibit human osteosarcoma MG-63 cells,it has certain anti-tumor activity in vitro,and dandelion ethyl acetate extract is particularly the most effective.

关 键 词：骨肉瘤 MG-63传代细胞 萃取物 蒲公英 

分 类 号：R965.1[医药卫生—药理学]