GNA15对重型哮喘患儿预后的预测价值  

Expression of GNA15 in peripheral blood in children with severe asthma and its prognostic value

作　　者：李丹[1] 范宇杭 李耕旭 陈新春[1] 孙国华[1] 李世峰 LI Dan;FAN Yuhang;LI Gengxu;CHEN Xinchun;SUN Guohua;LI Shifeng(Department of Pediatric,Tangshan People′s Hospital,Tangshan 063001,China;不详)

机构地区：[1]唐山市人民医院儿科,河北唐山063001 [2]华北理工大学公共卫生学院,河北唐山063210 [3]华北理工大学临床医学院,河北唐山063210

出　　处：《实用医学杂志》2022年第6期758-762,共5页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:82003406);河北省自然科学基金(编号:H2020209287);唐山市科技计划项目(编号:20130206b)。

摘　　要：目的 研究GNA15在重型哮喘(severe asthma,SA)患儿外周血中的表达及其预后价值。方法 数据集GSE27011下载于美国国立生物信息中心的基因表达数据库(GEO),通过生物信息学分析获得关键基因GNA15。收集儿童的病例资料和血液样本,检测外周血GNA15、炎症因子IL-4和IL-17的表达水平和呼吸功能指标,进行重型哮喘患儿预后评估的单因素和多因素分析,采用受试者工作特征曲线(ROC)评估血液GNA15预测重型哮喘患儿不良预后的效能。结果 GNA15在对照组、轻度哮喘组和重型哮喘组患儿外周血单个核细胞中表达逐渐升高;单因素分析结果显示,GNA15是重型哮喘组患儿预后不良的独立风险因素(OR=5.117);GNA15重型哮喘不良预后具有较好的预测效能(AUC=0.820,灵敏度为0.733,特异性为0.879,准确度为0.820)。结论 GNA15在重型哮喘患儿外周血中高表达且可预测重型哮喘患儿预后不良。Objective To investigate the expression of GNA15 in peripheral blood in children with severe asthma(SA)and its prognostic value.Methods The dataset GSE27011 was downloaded from the Gene Expression Omnibus(GEO)of the National Center for Biotechnology Information in the United States.The key gene GNA15was obtained by bioinformatics analysis.The medical records and blood samples of children were collected,and the expression levels of GNA15,inflammatory factors IL-4 and IL17 in peripheral blood,and respiratory function indexes were detected.The obtained data were used for univariate and multivariate analysis on prognosis assessment of children with SA.The receiver operating characteristic curve(ROC)was used to evaluate the efficacy of blood GNA15 in predicting SA children with poor prognosis.Results The expressions of GNA15 in peripheral mononuclear cells were gradually increased in the control group,MA group and SA group.The results of univariate analysis showed that GNA15 was an independent risk factor for poor prognosis in SA children(OR=5.117).GNA15 had a good efficacy in predicting poor prognosis of SA,with a sensitivity of 0.733,specificity of 0.879,and accuracy of 0.820.Conclusions GNA15 was highly expressed in peripheral blood,predicting a poor prognosis in children with SA.

关 键 词：儿童哮喘 重型哮喘 Gα蛋白15 受试者工作特征曲线 预后 

分 类 号：R56[医药卫生—呼吸系统]