初烤和复烤对烟叶真菌群落结构的影响  

Effects of flue-curing and redrying on fungal community structure of tobacco leaves

作　　者：成瑜 刘京 高英明 周家喜[1] 芶剑渝 管景强 邹晓[1,2] CHENG Yu;LIU Jing;GAO Yingming;ZHOU Jiaxi;GOU Jianyu;GUAN Jingqiang;ZOU Xiao(Institute of Fungus Resources,College of Life Sciences,Guizhou University,Guiyang 550025,China;Zunyi Branch of Guizhou Provincial Tobacco Company,Zunyi 563000,Guizhou,China;Institute of Agro-bioengineering,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学生命科学学院真菌资源研究所,贵阳市550025 [2]贵州省烟草公司遵义市公司,贵州省遵义市563000 [3]贵州大学农业生物工程研究院,贵阳市550025

出　　处：《烟草科技》2022年第5期9-16,共8页Tobacco Science & Technology

基　　金：贵州省烟草公司遵义市公司科技项目“提高烟叶油分含量的真菌生态制剂研发与示范”(遵烟计[2018]11号);贵州省烟草公司遵义市公司科技项目“应用环链棒束孢生态防控烟田小地老虎”(遵烟计[2021]5号)。

摘　　要：为了解初烤和复烤环节对烟叶真菌群落结构的影响,以贵州湄潭、平坝和惠水的烟叶为研究对象,通过高通量测序技术分析不同加工阶段(初烤前、初烤后、复烤前和复烤后)烟叶的真菌群落。12个样本共检测到真菌界4个门、23个纲、71个目、196个科、445个属、724个种和1171个OTUs。结果表明初烤和复烤前后的烟叶真菌组成存在明显差异:在属水平上,初烤前Sampaiozyma和Symmetrospora为优势属,其相对丰度分别为44.27%和21.24%;初烤后Sampaiozyma、链格孢属(Alternaria)和未分类的亚隔孢壳科(Didymellaceae)真菌为优势属,其相对丰度分别为37.87%、18.42%和12.89%;复烤前曲霉属(Aspergillus)、Sampaiozyma和根霉属(Rhizopus)为优势属,其相对丰度分别为60.82%、24.12%和2.51%;复烤后Sampiozyma和曲霉属(Aspergillus)为优势属,其相对丰度分别为46.12%和25.78%。Sampaiozyma在4个加工阶段一直占据优势地位。To investigate the effects of flue-curing and redrying on fungal community structure of tobacco leaves,tobacco leaf samples were collected from Meitan,Pingba and Huishui in Guizhou Province.The fungal community structure of the samples at different processing stages(before flue-curing,after flue-curing,before redrying and after redrying)was analyzed by high-throughput sequencing technology.A total of 4 phyla,23 classes,71 orders,196 families,445 genera,724species and 1171 OTUs were detected from 12 leaf samples.The results showed that there were significant differences in the composition of tobacco fungi before and after flue-curing or redrying:at the genus level,Sampaiozyma and Symmetrospora were the dominant genera before flue-curing,and their relative abundances were 44.27% and 21.24% respectively.After flue-curing,Sampaiozyma,Alternaria and unclassified Didymellaceae were the dominant genera with relative abundances of 37.87%,18.42% and 12.89% respectively.Before redrying,Aspergillus,Sampaiozyma and Rhizopus were the dominant genera with relative abundances of 60.82%,24.12% and 2.51%respectively;after redrying,Sampiozyma and Aspergillus were the dominant genera with relative abundances of 46.12% and 25.78%respectively.Sampaiozyma were the dominant fungi at all four processing stages.

关 键 词：烟叶 初烤 复烤 真菌群落 烟叶防霉 高通量测序 

分 类 号：TS44[农业科学—烟草工业]