Mechanism of Fuzheng Kang'ai Formula Regulating Tumor Microenvironment in Non-Small Cell Lung Cancer  

作　　者：TIAN Yun-ling FU Song-bo LI Bo YUAN Ling-yan BING Zhi-tong 

机构地区：[1]Department of Endocrinology and Metabolism,the First Hospital of Lanzhou University,Lanzhou(730000),China [2]Evidence Based Medicine Center,School of Basic Medical Science of Lanzhou University,Lanzhou(730000),China [3]Department of Computational Physics,Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou(730000),China [4]School of Nuclear Science and Technology,University of Chinese Academy of Sciences,Beijing(100049),China

出　　处：《Chinese Journal of Integrative Medicine》2022年第5期425-433,共9页中国结合医学杂志（英文版）

基　　金：Supported by the Special Funds of Science and Technology Development of the Chinese Central Government to Guide Local in 2020(No.21JR1RA066,20JR10FA667);Hospital Fund of the First Hospital of Lanzhou University(No.ldyyyn2007-03)。

摘　　要：Objective:To study the mechanism of Chinese herbal medicine Fuzheng Kang’ai Formula(FZKA)on tumor microenvironment(TME).Methods:CIBERSORTx was used for analysis of TME.Traditional Chinese Medicine Systems Pharmacology and Analysis Platform was applied to identify compounds-targets network and the Cancer Genome Atlas(TCGA)was employed to identify the differential expression genes(DEGs)between tumor and paracancerous tissues in lung adenocarcinoma(LUAD)from TCGA-LUAD.Additionally,DEGs with prognosis in LUAD was calculated by univariable and multivariate Cox regression.The core targets of FZKA were analyzed in lung adenocarcinoma TME.Protein-protein interaction database was employed to predict down-stream of target.Quantitative reverse transcription polymerase chain reaction was employed for biological experiment in A549,H1299 and PC9 cell lines.Results:The active and resting mast cells were significantly associated with prognosis of LUAD(P<0.05).Of the targets,CCNA2 as an important target of FZKA(hazard ratio=1.41,95%confidential interval:1.01-2.01,P<0.05)was a prognostic target and significantly associated with mast cells.CCNA2 was positively correlated with mast cell activation and negatively correlated with mast cell resting state.BCL1 L2,ACTL6 A and ITGAV were down-stream of CCNA2,which were validated by q RT-PCR in A549 cell.Conclusion:FZKA could directly bind to CCNA2 and inhibit tumor growth by regulating CCNA2 downstream genes and TME of NSCLC closely related to CCNA2.

关 键 词：network pharmacology PROGNOSIS tumor microenvironment Fuzheng Kang’ai Formula Chinese medicine lung adenocarcinoma 

分 类 号：R285[医药卫生—中药学]