脑震宁对脑震荡后综合征模型大鼠海马线粒体功能的影响  

Effect of Naozhenning on Hippocampal Mitochondrial Function in Model Rats with Post-concussion Syndrome

作　　者：江晓智 赵乐 王钱 谭贝西 王超群 王永辉 JIANG Xiao-zhi;ZHAO Le;WANG Qian;TAN Bei-xi;WANG Chao-qun;WANG Yong-hui(Shanxi University of Chinese Medicine,Jinzhong 030619,China;Hubei University of Chinese Medicine,Wuhan 430065,China)

机构地区：[1]山西中医药大学,山西晋中030619 [2]湖北中医药大学,武汉430065

出　　处：《中国实验方剂学杂志》2022年第11期86-92,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(8210151374);山西中医药大学科研启动基金项目(2021BKS03)。

摘　　要：目的:基于线粒体功能,探究脑震宁对脑震荡后综合征模型大鼠学习记忆能力及海马CA1区神经元损伤的作用机制。方法:SPF级Wistar大鼠,采用自由落体撞击法制备三重脑震荡后综合征模型(MCC),将造模成功的大鼠按随机数字表法分成5组,模型组、吡拉西坦组、脑震宁低、中、高剂量组,另设正常组,每组10只。吡拉西坦组及脑震宁低、中、高剂量组分别以0.43、5.4、10.8、21.6 g·kg^(-1)的剂量灌胃给药,正常组和模型组大鼠分别灌服等体积蒸馏水,连续给药14 d进行相关指标检测。采用Morris水迷宫实验观察各组大鼠逃避潜伏期、原象限停留时间及穿越平台次数等学习记忆改变情况;苏木素-伊红(HE)染色、尼氏染色观察海马CA1区病理形态学改变;透射电子显微镜(TEM)观察线粒体超微结构;比色法检测线粒体呼吸链复合体Ⅰ活性;荧光探针法测定腺嘌呤核苷三磷酸(ATP)含量;酶联荧光免疫技术法检测线粒体膜电位(MMP)。结果:与正常组比较,模型组大鼠逃避潜伏期时间明显延长,目标象限停留时间及穿越平台次数明显减少,海马CA1区神经元和尼氏小体数量减少,神经元形态及线粒体结构遭受破坏,线粒体膜电位、ATP和呼吸链复合体Ⅰ含量明显减少(P<0.05,P<0.01);与模型组比较,脑震宁各剂量组大鼠逃避潜伏期明显缩短,目标象限停留时间及穿越平台次数明显增加,海马CA1区神经元及尼氏小体数量显著增加,神经元形态及线粒体结构损伤有所改善,线粒体膜电位、ATP和呼吸链复合体Ⅰ含量明显增加(P<0.05,P<0.01),脑震宁低剂量组大鼠穿越平台次数及线粒体呼吸链复合体Ⅰ差异无统计学意义。结论:脑震宁可以改善MCC大鼠学习记忆能力,其机制可能与改善线粒体结构与功能,减轻海马神经元损伤有关。Objective:To explore the mechanism of Naozhenning on learning and memory ability and neuron damage in hippocampal CA1 region of post-concussion syndrome model rats based on mitochondrial function.Method:Multiple cerebral concussion(MCC)was induced in SPF Wistar rats with the free-fall impact method.Then the model rats were randomly classified into model group(equivalent volume of distilled water),piracetam(0.43 g·kg^(-1),ig)group,and low-,medium-,and high-dose NZN(5.4,10.8,21.6 g·kg^(-1),respectively,ig)groups,with 10 rats in each group,and another 10 normal rats were included in the normal control group(equivalent volume of distilled water).The administration lasted 14 days and then relevant indexes were detected.Morris water maze test was used to observe the changes of learning and memory ability in each group,such as escape latency,residence time in primary quadrant,and times of crossing platform.The pathological changes of hippocampal CA1 region were observed based on hematoxylin-eosin(HE)staining and Nissl staining.The ultrastructure of mitochondria was observed under the transmission electron microscope(TME)and the activity of mitochondrial respiratory chain complexⅠwas detected by colorimetry.The content of adenosine triphosphate(ATP)was determined by fluorescence probe and mitochondrial membrane potential(MMP)by fluorescein enzyme-linked fluorescence immunoassay.Result:Compared with the normal control group,the model group showed long escape latency,short residence time in target quadrant,few times of crossing the platform,significant decrease in counts of neurons and Nissl bodies in hippocampal CA1 region,damage of neuronal morphology and mitochondrial structure,and significant reduction of MMP and the content of mitochondrial ATP and respiratory chain complex I(P<0.05,P<0.01).The NZN groups demonstrated short escape latency,long residence time in target quadrant,increased times of crossing the platform,small number of neurons and Nissl bodies in hippocampal CA1 region,alleviated damage of neuronal m

关 键 词：脑震宁 脑震荡后综合征 海马 线粒体功能 学习记忆 

分 类 号：R2-0[医药卫生—中医学] R22