西瑁岛软珊瑚Dendronephthya sp.的化学成分和生物活性研究  

Chemical constituents and bioactivities of soft coral Dendronephthya sp.from Ximao Island

作　　者：李旺盛 高天润 章海燕 郭跃伟 罗辉 刘进 LI Wang-sheng;GAO Tian-run;ZHANG Hai-yan;GUO Yue-wei;LUO Hui;LIU Jin(School of Pharmacy,Guangdong Medical University,Zhanjiang 524023,China;State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China)

机构地区：[1]广东医科大学药学院,广东湛江524023 [2]中国科学院上海药物研究所新药研究国家重点实验室,上海201203

出　　处：《中国海洋药物》2022年第2期7-13,共7页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金重大项目(81991521)资助。

摘　　要：目的 对采自中国南海西瑁岛海域的软珊瑚Dendronephthya sp.的化学成分和抗炎活性进行研究。方法 综合利用薄层色谱、硅胶柱色谱和凝胶柱层析等分离手段,对纯化的单体成分采用核磁波谱(NMR)和质谱(MS)等方法进行鉴定,结合文献数据比对确定化合物结构;并对化合物进行了抗炎活性测试和细胞毒性实验。结果 从软珊瑚Dendronephthya sp.中共分离鉴定了11个化合物,包括6个甾体化合物,4个嘧啶类化合物及1个醇类化合物:胆固醇(1),胆固醇乙酸脂(2),胆固醇-5α, 6α-环氧化物(3),鲨肝醇(4),11-acetoxy-3β,6α-dihydroxy-9, 11-seco-5α-cholest-7-en-9-one(5),啤酒甾醇(6),3β, 5α, 6β-三羟基胆甾烷醇(7),胸腺嘧啶(8),脲嘧啶(9),胸苷(10),脲苷(11)。结论 化合物5和6是首次从该种属中分离得到,其中化合物5对脂多糖诱导的BV-2细胞的炎症反应具有抑制作用。Objective To study the chemical constituents and bioactivities of soft coral Dendronephthya sp. collected from Ximao Island in South China Sea. Methods The isolation and purification of these compounds were performed by TLC, silica gel and Sephadex LH-20 column chromatography. Their structures were characterized by analysis of NMR and MS data, as well as by comparison with the data reported in literature and the anti-inflammatory activities of the pure compounds were tested. Results Eleven compounds were isolated from the soft coral Dendronephthya sp. and their structures were identified as cholesterol(1), cholesterol acetate(2), cholesterol-5α, 6α-Epoxide(3), squaliol(4), 11-acetoxy-3β, 6α-dihydroxy-9, 11-seco-5α-cholest-7-en-9-one(5), beer sterol(6), 3β, 5α, 6β-trihydroxycholestanol(7), thymine(8), uracil(9), thymidine(10), uridine(11). Conclusion Compounds 5 and 6 were first isolated from the genus coral, of which compound 5 could inhibit lipopolysaccharide induced inflammation in BV-2 microglial cells.

关 键 词：软珊瑚 甾体 细胞毒性 抗炎 

分 类 号：R931[医药卫生—生药学]