仿刺参糖胺聚糖对肺结核和肺癌患者免疫调节功能研究  

The research of Glycosaminoglycan from Apostichopus japonicus on immune regulation function in patients with pulmonary tuberculosis and lung cancer

作　　者：于丁一 朱敬森 肖宝红 丁海升 林存智[1] YU Ding-yi;ZHU Jing-sen;XIAO Bao-hong;DING Hai-sheng;LIN Cun-zhi(Department of Respiratory&Critical Care Medicine,The Affiliated Hospital of Qingdao University,Qingdao 266003,China;Department of Respiratory Medicine,Qingdao west coast new area district hospital,Qingdao 266555,China)

机构地区：[1]青岛大学附属医院呼吸与危重症医学科,山东青岛266003 [2]青岛市西海岸新区区立医院呼吸与危重症医学科,山东青岛266555

出　　处：《中国海洋药物》2022年第2期71-75,共5页Chinese Journal of Marine Drugs

基　　金：海洋药物教育部重点实验室科研基金项目(KLMDOUC201307);青岛西海岸新区科技局课题(2019-52)资助。

摘　　要：目的 探讨仿刺参糖胺聚糖(HGAG)对肺结核和肺癌患者免疫调节功能影响。方法 选取健康人群40名(健康组),肺结核患者30例(结核组)和肺癌患者30例(肺癌组),取抗凝外周血4 mL,采用Ficoll密度梯度离心法分离外周血单个核细胞(PBMC),体外与不同浓度HGAG共培养24 h。运用流式细胞仪检测CD2、CD28和B7(B7-1和B7-2)分子的表达。结果 健康组CD2、CD28和B7-2表达在10μg·mL;最明显,而B7-1在50μg·mL^(-1)最明显,与对照组比较差异均具有统计学意义(P<0.05);结核组CD2、CD28和B7-1表达在50μg·mL^(-1)最明显,B7-2以10μg·mL^(-1)表达最明显,与对照组比较差异均具有统计学意义(P<0.05);肺癌组CD2、CD28、B7在100μg·mL^(-1)时表达最明显,与对照组比较差异均具有统计学意义(P<0.05)。结论 HGAG在一定浓度范围内可以不同程度促进CD2、CD28和B7分子表达,体外调节肺结核和肺癌患者的细胞免疫功能。Objective To study the effect of Apostichopus japonicus glycosaminoglycan(HGAG) on immune regulation in patients with pulmonary tuberculosis and lung cancer. Methods 40 healthy people(healthy group), 30 pulmonary tuberculosis patients(tuberculosis group) and 30 lung cancer patients(lung cancer group) were selected and anticoagulation peripheral blood 4 mL were taken. The peripheral blood mononuclear cells(PBMC) were isolated by Ficoll density gradient centrifugation, and cultured in vitro with different concentrations of HGAG for 24 h. The expressions of CD2, CD28 and B7(B7-1 and B7-2) molecules were detected by flow cytometry respectively. Results The expression levels of CD2, CD28 and B7-2 in healthy group were the most obvious at 10 μg·mL^(-1), while the expression levels of B7-1 were the most obvious at 50 μg·mL^(-1), which were both different from those in control group(P<0.05). The expression of CD2, CD28 and B7-1 in tuberculosis group were the most obvious at 50 μg·mL^(-1), while the expression levels of B7-2 were the most obvious at 10 μg·mL^(-1), which were both different from those in control group(P<0.05). The expression of CD2, CD28 and B7 in lung cancer group was the most obvious at 100 μg·mL;, which was different from that in control group(P<0.05). Conclusion HGAG could promote the expression of CD2, CD28 and B7 molecules in varying degrees, regulating the cellular immune function of pulmonary tuberculosis and lung cancer patients in vitro.

关 键 词：仿刺参糖胺聚糖 肺结核 肺癌 外周血单个核细胞 细胞免疫调节 

分 类 号：R931.77[医药卫生—生药学]