新型生物色谱固定相制备及分析方法学研究进展  

Advances in methodologies for preparation and analysis of new biochromatic stationary phase

作　　者：柴心怡 顾妍秋 陈啸飞 柴逸峰 CHAI Xinyi;GU Yanqiu;CHEN Xiaofei;CHAI Yifeng(School of Pharmacy,Naval Medical University,Shanghai 200433,China;Department of Pharmacy,Ninth People's Hospital Affiliated to Shanghai Jiaotong University,Shanghai 201999,China)

机构地区：[1]海军军医大学药学院,上海200433 [2]上海交通大学医学院附属第九人民医院,上海201999

出　　处：《药学实践杂志》2022年第3期193-198,共6页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金项目(82073814、81973291、82122066、82003909);上海市青年科技启明星计划项目(19QA1411500)。

摘　　要：生物色谱法是一种极具发展潜力的新兴色谱技术,已广泛用于药物筛选及生物分子间相互作用分析。其技术核心是生物分子的色谱固定相,现今主要发展了细胞膜色谱,人工仿生膜色谱以及通过开发多种固定化策略将蛋白等直接固定于固定相载体。本文对新型生物色谱固定相方法学研究进展及基于新型固定相的生物色谱分析应用研究现状进行综述,并展望了基于整体柱的生物色谱固定相及微型生物色谱分析系统的应用前景。Biochromatography is a new chromatographic technology with great development potential.It has been widely used in drug screening and biomolecular interaction analysis.The core of this technology is the chromatographic stationary phase of biomolecules.Nowadays,it mainly develops cell membrane chromatography,artificial biomimetic membrane chromatography and the various immobilization strategies to directly immobilizes proteins on the stationary phase carrier.This paper reviews the research progress of new biochromatographic stationary phase and the application of biochromatographic analysis based on new stationary phase.And,the applications of biochromatographic stationary phase and micro biochromatographic analysis system based on monolithic column are prospected.

关 键 词：生物色谱 固定化方法 药物筛选 整体柱 

分 类 号：R917[医药卫生—药物分析学]