光照对小麦的春化效应及其机制初探  

Effect of Light on the Regulation of Vernalization in Wheat(Triticum aestivum L.)and Its Preliminary Mechanism

作　　者：张洪伟 焦义然 陈文烨 董福双[2] 杜进民[3] 王海波 周硕[2] ZHANG Hongwei;JIAO Yiran;CHEN Wenye;DONG Fushuang;DU Jinmin;WANG Haibo;ZHOU Shuo(College of Agronomy, Hebei Agricultural University, Baoding, Hebei 071001, China;Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences/Plant Genetic Engineering Center of Hebei Province,Shijiazhuang, Hebei 050051, China;College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China)

机构地区：[1]河北农业大学农学院,河北保定071001 [2]河北省农林科学院生物技术与食品科学研究所/河北省植物转基因中心,河北石家庄050051 [3]河北科技大学食品与生物学院,河北石家庄050051

出　　处：《麦类作物学报》2022年第4期433-440,共8页Journal of Triticeae Crops

基　　金：河北省现代农业科技创新工程项目(2019-4-8-1);河北省重点研发计划项目(20326306D)。

摘　　要：为解析光照对春化过程的影响及其机制,以中麦175为材料,比较了光照春化及暗春化28 d时该品种开花时间的差异,并对春化前、春化7 d、14 d、21 d和28 d时,两种春化条件下小麦叶片中春化及光周期相关基因进行了real-time PCR分析。结果表明,与暗春化相比,光照春化使小麦提前开花19.73 d;与春化、光周期相关的基因(TaVRN1、TaVRN2、TaVER2、TaVIL1、TaVIL2、TaVIL3、TaCO1、TaCO2、TaPPD1、TaGI1、TaLHY和TaTOC1)在光照春化条件下的表达水平较高,这可能是光照春化导致小麦提早开花的主要原因之一,也说明小麦中可能存在未知的TaVRN1调控因子,这些调控因子同时受到光照和低温条件的调控。In order to analyze the effect of light on the regulation of vernalization and its mechanism,the flowering time of a wheat cultivar Zhongmai 175 after vernalization in light or dark for 28 d were compared.The leaf samples were also collected during vernalization in light or dark for 0 d,7 d,14 d,21 d and 28 d,and real-time PCR analysis of vernalization-and photoperiod-related genes was performed using these samples.The result showed that compared to dark vernalization,vernalization with light brought anthesis forward by 19.73 days in wheat.The real-time PCR revealed an elevated expression for most of the vernalization and photoperiod related genes(TaVRN1,TaVRN2,TaVER2,TaVIL1,TaVIL2,TaVIL3,TaCO1,TaCO2,TaPPD1,TaGI1,TaLHY and TaTOC1)analyzed in this study under vernalization in light.This may be one of the major reasons for early flowering under vernalization in light in wheat.These results also suggest some hitherto unknown regulatory factors relate to TaVRN1,regulating both by light and low temperature.

关 键 词：小麦 春化 光照 基因表达 

分 类 号：S512.1[农业科学—作物学] S330