基于“精华与糟粕分解”理论的幽门结扎性肝损伤病因及连翘-4保肝作用物质基础研究  

Study on the Etiology of Pyloric Ligation Liver Injury Based on the“Essence and Dregs Decomposition”Theory and the Material Basis of Forsythia-4Hepatoprotective Effect

作　　者：苓苓 昂格力玛 宫菊花 吴圆圆 杜兰 王欢[1] Ling Ling;Ang Gelima;Gong Juhua;Wu Yuanyuan;Du Lan;Wang Huan(College of Mongolian Medicine and Pharmacy,Inner Mongolia University for Nationalities,Tongliao 028000,China)

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古通辽028000

出　　处：《亚太传统医药》2022年第5期32-35,共4页Asia-Pacific Traditional Medicine

基　　金：国家自然科学基金(82060910)。

摘　　要：目的:研究含药血清对LO2肝细胞的影响,探索幽门结扎性肝损伤的病因及连翘-4保肝作用物质基础。方法:通过动物实验制备幽门结扎性肝损伤大鼠门静脉血清和含连翘-4血清。采用体外培养LO2肝细胞实验,观察幽门结扎性肝损伤大鼠门静脉血清及含药血清(连翘-4)对肝细胞的影响。结果:与正常组比较,模型组门静脉血清LO2肝细胞上清液AST、ALT水平明显上升(P<0.01),并在镜下观察发现,正常组细胞生长状态良好,大小均匀,边界清楚;模型组细胞数量明显减少,细胞明显融合。模型组门静脉血清与连翘-4含药血清比较,细胞上清液AST、ALT与镜下观察均未发现明显变化。结论:该研究初步判断为幽门结扎致肝损伤的病理过程,有可能是因胃内“精华与糟粕分解”紊乱,致受影响的食物精华通过门静脉输送至肝脏,从而导致肝功能异常。但由于实验方法与测量误差等方面的原因,从连翘-4腹主动脉血清内未发现保肝作用的有效物质基础,因此对该方药保护幽门结扎性肝损伤有效物质基础还需进一步研究。Objective:To explore the etiology of pyloric ligated liver injury and the material basis of forsythia-4’s liverprotecting effect by studying the effect of drug-containing serum on LO2liver cells.Methods:The portal vein serum and forsythia-4serum of rats with pyloric ligation liver injury were prepared by animal experiment.The effects of portal vein serum and drug-containing serum(forsythia-4)on hepatocytes of rats with pyloric ligation liver injury were observed by LO2hepatocytes cultured in vitro.Results:Compared with normal group,AST and ALT levels in the supernatant of LO2hepatocytes in portal vein serum of model group were significantly increased(P<0.01).Microscope observation showed that cells in normal group were in good growth state,uniform size and clear boundary.The number of cells in the model group was significantly reduced and the cells were fused.There were no significant changes in AST and ALT in the cell supernatant between model group and forsythia-4drug containing serum.Conclusion:Through this study,it is preliminarily concluded that the pathological process of liver injury caused by pyloric ligation may be caused by the disorder of“essence and dregs decomposition”in the stomach,which leads to the delivery of the affected food essence to the liver through the portal vein,resulting in abnormal liver function.However,due to the experimental methods and measurement errors,no effective material basis for protecting liver was found in the serum of Forsythia-4 abdominal aorta.Therefore,further exploration is needed on the effective material basis of this formula for protecting pyloric ligated liver injury.

关 键 词：“精华与糟粕分解”理论 幽门结扎性肝损伤 连翘-4保肝作用物质基础 

分 类 号：R256.4[医药卫生—中医内科学]