基原为云南独蒜兰的山慈菇的化学成分鉴定与分析  

Identification and analysis of chemical constituents of Pleione yunnanensis with origin of Pleione yunnanensis

作　　者：王静[1,2] 肖秋肖 周祖英 仁莎莎 陈思颖 巩仔鹏[1] 黄勇[1] 王爱民[3] 刘春花[3] 李月婷[1] WANG Jing;XIAO Qiuxiao;ZHOU Zuying;REN Shasha;CHEN Siying;GONG Zipeng;HUANG Yong;WANG Aimin;LIU Chunhua;LI Yueting(Provincial Key Laboratory of Pharmaceutics in Guizhou/State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550004,China;School of Pharmacy,Guizhou Medical University,Guiyang 550004,China;Engineering Research Center of the Minstry of Education for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine,Guizhou Medical University,Guiyang 550004,China)

机构地区：[1]贵州医科大学贵州省药物制剂重点实验室/省部共建药用植物功效与利用国家重点实验室,贵阳550004 [2]贵州医科大学药学院,贵阳550004 [3]贵州医科大学民族药与中药开发应用教育部工程研究中心,贵阳550004

出　　处：《中国药房》2022年第10期1165-1171,共7页China Pharmacy

基　　金：国家自然科学基金委-特色民族药药效物质及体内过程与毒效机制研究(No.U1812403-5);中央引导地方科技发展专项资金项目(No.黔科中引地[2018]4006);贵州省高层次创新型人才项目[No.黔科合平台人才(2016)5677]。

摘　　要：目的鉴定、分析山慈菇(基原为云南独蒜兰)的化学成分。方法采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱联用技术。色谱柱为Hyperdil GOLD,流动相为0.1%甲酸溶液-0.1%甲酸乙腈溶液(梯度洗脱),流速为0.3 mL/min,柱温为40℃,进样量为2μL;离子源为电喷雾离子源,扫描范围为m/z 100~1500,扫描模式为全扫描+数据依赖二级扫描的正负离子交换模式。采用Compound Discoverer 3.1软件,通过与mzCloud、PubChem网络数据库和中药成分高分辨质谱本地数据库OTCML进行比对,同时结合对照品和已有文献确定化合物结构。结果与结论从山慈菇中共鉴定出42个化学成分(正离子模式下有24个、负离子模式下有27个),包括13个丁二酸苄酯苷类成分(如dactylorhin C、coelovirin A、militarine等)、4个酚苷类成分(如腺苷、鸟苷、天麻素等)、3个生物碱类成分(胆碱、甜菜碱、小檗碱)、1个黄酮类成分(川陈皮素)、7个芳香族类成分(如DL-赖氨酸、DL-精氨酸、DL-谷氨酰胺等)、1个糖类成分(蔗糖)、3个联苄类成分(shancigusin H、shancigusin H isomer、山药素Ⅲ)和10个其他类成分(如对甲氧基苯甲酸、十二烷二酸单甲酯、二苯胺等)。丁二酸苄酯苷类成分在裂解过程中易丢失葡萄糖氧苄基和一些小分子;酚苷类成分易丢失糖基;生物碱类成分以小分子取代基的裂解和丢失为主;黄酮类成分多发生脱甲基反应。OBJECTIVE To identify and analyze chemical constituents of Pleione yunnanensis with origin of Pleione yunnanensis.METHODS UPLC-Q-Exactive-Plus-Orbitrap-MS was adopted.The determination was performed on Hyperdil GOLD column with mobile phase consisted of 0.1%formic acid solution-0.1%formic acid acetonitrile solution(gradient elution)at the flow rate of 0.3 mL/min.The column temperature was set at 40℃,and sample size was 2μL.The electrospray ion source was adopted,and the scanning range was m/z 100-1500,and the scanning mode was positive and negative ion exchange mode of full scan+ddMS2.The structure of chemical constituents were determined by using Compound Discoverer 3.1 software,comparing with mzCloud,PubChem network database and OTCML,on the basis of reference substance and published literatures.RESULTS&CONCLUSIONS A total of 42 chemical constituents were identified(positive ion mode has 24,negative ion mode has 27),including 13 benzyl succinate glycosides(such as dactylorhin C,coelovirin A,militarine),4 phenol glycosides(such as adenosine,guanosine,gastrodin),3 alkaloids(choline,betaine,berberine),and one flavonoid(nobiletin),7 aromatics(such as DL-lysine,DL-arginine,DL-glutamine),one sugar(sucrose),3 benzenes(shancigusin H,shancigusin H isomer,batatasinⅢ)and 10 others(such as p-methoxybenzoic acid,monomethyl dodecanedioate,diphenylamine).Glucose oxybenzyl and some small molecules are easy to be lost in the cleavage of benzyl succinate glycosides;glycosyl is easy to be lost in the cleavage of phenol glycosides;the cleavage of alkaloids mainly manifest as the cleavage and loss of small molecular substituents;demethyla-tion reaction is occurred in most flavonoids.

关 键 词：山慈菇 超高效液相色谱-四极杆-静电场轨道阱高分辨质谱联用技术 化学成分 鉴定 分析 

分 类 号：R917[医药卫生—药物分析学] R284.1[医药卫生—药学]