灵芝三萜提取工艺优化  

Optimization of Extraction Technology of Triterpenoid from Ganoderma lucidum

作　　者：郑苗欣 张振伟 王雪婷 周伟坚[1] 莫转林 莫美华[1] ZHENG Miao-xin;ZHANG Zhen-wei;WANG Xue-ting;ZHOU Wei-jian;MO Zhuan-lin;MO Mei-hua(College of Food Science and Technology,South China Agricultural University,Guangzhou 510642,China;Experimental Basis and Practical Training Center,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]华南农业大学基础实验与实践训练中心,广东广州510642

出　　处：《中国食用菌》2022年第4期64-68,共5页Edible Fungi of China

基　　金：广东省科技创新战略专项资金(重点领域研发计划项目)(2018B020206001);广东省农业科研项目和农业技术推广项目(2021KJ103)。

摘　　要：以灵芝T0菌株的菌丝体为原料,采用超声辅助提取三萜,在单因素试验结果的基础上,通过正交试验得到优化最佳工艺条件。结果表明,在乙醇浓度85%、提取时间30 min、料液比1∶30的条件下,灵芝菌丝体三萜的平均得率达到了3.50%。该结果为优化灵芝菌丝体三萜的提取工艺提供了有利参考。Triterpenoids were extracted from Ganoderma lucidum mycelium by ultrasonic assisted extraction.Based on the results of single factor experiment,the optimal process conditions were obtained by orthogonal experiment.The results showed that ethanol concentration 85%,extraction time 30 min,solid-liquid ratio 1∶30,the average extraction rate of triterpenoid from G.lucidum mycelium reached 3.50%.The results provides a favorable reference for the optimization of the extraction process of triterpenoids from G.lucidum mycelium.

关 键 词：灵芝 菌丝体 三萜 正交试验 提取工艺优化 

分 类 号：S646.2[农业科学—蔬菜学]