组蛋白去乙酰化酶3、TGF⁃β1在狼疮肾炎组织病理中的表达及其临床意义  

Expression of histone deacetylase 3 and TGF⁃β1 in histopathology of lupus nephritis and its clinical significance

作　　者：史添立[1] 罗贞[1] 焦石[2] SHI Tianli;LUO Zhen;JIAO Shi(Department of Nephrology,Haikou Municipal People's Hospital,Haikou,Hainan,China,570208;Department of Nephrotic Rheumatology,Zhongshan City Boai Hospital,Zhongshan,Guangdong,China,510603)

机构地区：[1]海口市人民医院肾内科,海南海口570208 [2]中山市博爱医院肾病风湿科,广东中山510603

出　　处：《分子诊断与治疗杂志》2022年第4期606-609,613,共5页Journal of Molecular Diagnostics and Therapy

基　　金：海南省卫生计生行业科研项目(19A200052)。

摘　　要：目的探讨组蛋白去乙酰化酶3(HDAC3)、转化生长因子⁃β1(TGF⁃β1)在系统性红斑狼疮患者肾脏病理中的表达及其临床意义。方法选取2017年6月至2020年9月海口市人民医院诊治的53例系统性红斑狼疮患者为SLE组;及10例原发性肾病综合征(NS)为对照组。比较两组患者肾脏病理组织中的表达情况,分析其表达水平与狼疮肾炎(LN)患者的各种临床表现、实验室特征以及肾脏病理类型的关系。应用t检验及单因素方差分析进行组间比较,Pearson或Spearman检验进行相关分析。结果HDAC3主要表达于肾脏肾小管上皮细胞中。SLE组患者肾组织中HDAC3的表达水平高于对照组[(6.32±2.31)vs(3.12±1.81)],差异有统计学意义(P<0.05)。伴有肾间质纤维化的LN患者HDAC3表达高于无纤维化患者[(6.78±2.19)vs(2.43±2.01)],差异有统计学意义(P<0.05)。肾脏组织HDAC3、TGF⁃β1表达均与血肌酐、浆膜炎、口腔溃疡、关节炎、间质性肺炎、神经系统损害、24小时尿蛋白及血液系统损害有关(P<0.05),肾小球组蛋白乙酰化酶3表达的水平与TGF⁃β1主要表达水平呈正相关(r=0.745,P<0.05)。结论LN患者的肾小球HDAC3,TGF⁃β1的表达水平较对照组增高,伴有肾间质纤维化的LN患者HDAC3表达高于无纤维化患者,考虑HDAC3可能参与LN患者纤维化的发病。Objective To investigate the expression of histone deacetylase 3(HDAC3)and TGF⁃β1 in renal tissues and clinical significance of patients with systemic lupus erythematosus(SLE).Methods Fifty⁃three patients with systemic lupus erythematosus diagnosed and treated in Haikou People's Hospital from June 2017 to September 2020 were selected as the SLE group;and 10 patients with primary nephrotic syndrome(NS)were selected as the control group.The expression in renal pathological tissue of the two groups of patients was compared,and the relationship between its expression level and various clinical manifestations,laboratory characteristics and renal pathological types of lupus nephritis(LN)patients was analyzed.The t test and one⁃way ANOVA were used for comparison between groups,and Pearson or Spearman test was used for correlation analysis.Results HDAC3 is mainly expressed in renal tubular epithelial cells.The expression level of HDAC3 in renal tissue of the SLE group was significantly higher than that of the control group(6.32±2.31 vs 3.12±1.81),(P<0.05).The expression of HDAC3 in LN patients with renal interstitial fibrosis was higher than that in patients without fibrosis(6.78±2.19)vs(2.43±2.01),(P<0.05).The expressions of HDAC3 and TGF⁃β1 in kidney tissue were related to serum creatinine,serositis,oral ulcers,arthritis,interstitial pneumonia,nervous system damage,24⁃hour urine protein and blood system damage(P<0.05).The expression level of histone acetylase 3 was positively correlated with the main expression level of TGF⁃β1(r=0.745,P<0.05).Conclusion The expression levels of HDAC3 and TGF⁃β1 in glomeruli of LN patients were higher than those in the control group,which were closely related to interstitial fibrosis of LN patients,suggesting that HDAC3 may be involved in the pathogenesis of LN patients with fibrosis.

关 键 词：系统性红斑狼疮 组蛋白去乙酰化酶3 转化生长因子⁃β 

分 类 号：R593.242[医药卫生—内科学]