焦化废水对菌藻颗粒污泥的微生物群落及代谢基因的影响  

Effect of Coking Wastewater on Microbial Communities and Metabolic Genes in Microalgae Bacteria Granular Sludge

作　　者：黄雪颖 陈威[1] 王旭 余哲 柯新语 王家乐 HUANG Xueying;CHEN Wei;WANG Xu;YU Zhe;KE Xinyu;WANG Jiale(School of Urban Construction,Wuhan University of Science&Technology,Wuhan 430081,China)

机构地区：[1]武汉科技大学城市建设学院,湖北武汉430081

出　　处：《河南科技大学学报（自然科学版）》2022年第5期100-104,M0008,共6页Journal of Henan University of Science And Technology:Natural Science

基　　金：国家自然科学基金项目(51808416);长江水利委员会长江科学院开放研究基金项目(CKWV2018490/KY)。

摘　　要：针对焦化废水毒性大、可生化性差的问题,利用菌藻颗粒污泥系统(MBGS)处理焦化废水。研究结果表明:MBGS对化学需氧量(COD)、氨氮(NH_(4)^(+)-N)和总无机氮(TIN)的去除率分别为74.01%、79.97%和57.39%。陶厄氏菌属(Thauera)和黄杆菌属(Flavobacterium)是优势菌属。通过宏基因组学分析发现基因ligA,说明MBGS对焦化废水的COD去除是通过苯环开环;基因nirB、nirD丰富度很高,说明MBGS对氨氮的去除是通过氨的解毒代谢,对无机氮的去除主要是通过异化硝酸盐还原作用。Aiming at the problems of high toxicity and poor biodegradability of coking wastewater,the microalgae bacteria granular sludge(MBGS)was used to treat coking wastewater.The results show that the removal rates of chemical oxygen demand(COD),ammonia nitrogen(NH_(4)^(+)-N)and total inorganic nitrogen(TIN)by MBGS are 74.01%,79.97%and 57.39%,respectively.Thauera and Flavobacterium are the dominant genus.GeneligAis found by metagenomic analysis,which indicate that COD removal from coking wastewater by MBGS is through benzene ring opening.The relative abundance of genesnirBandnirDis high,indicating that the removal of ammonia nitrogen by MBGS is through ammonia detoxification metabolism.The removal of inorganic nitrogen is mainly through dissimilatory nitrate reduction.

关 键 词：菌藻颗粒污泥 焦化废水 细菌群落结构 氮循环通路 

分 类 号：X592[环境科学与工程—环境工程]