电针干预对帕金森病模型小鼠黑质中酪氨酸羟化酶及氧化应激因子表达的影响  

Effects of Electroacupuncture Intervention on the Expression of Tyrosine Hydroxylase and Oxidative Stress Factor in Substantia Nigra of Parkinson’s Disease Model Mice

作　　者：赵颖倩 亢恺雯 刘奇 马雪 李杰 鲁刚 王强 ZHAO Yingqian;KANG Kaiwen;LIU Qi;MA Xue;LI Jie;LU Gang;WANG Qiang(School of Acupuncture-moxibustion and Tuina,Shaanxi University of Chinese Medicine,Xianyang 712046,China;The Second Clinical Medical School of Shaanxi University of Chinese Medicine,Xianyang 712046,China;Shaanxi Provincial Key Laboratory of Acupuncture Combined with Medication,Xianyang 712046,China;Xianyang Key Laboratory of Neurobiology(Acupuncture-moxibustion),Xianyang 712046,China)

机构地区：[1]陕西中医药大学针灸推拿学院,咸阳712046 [2]陕西中医药大学第二临床医学院,咸阳712046 [3]陕西省针药结合重点实验室,咸阳712046 [4]咸阳市神经生物学(针灸)重点实验室,咸阳712046

出　　处：《上海针灸杂志》2022年第5期521-527,共7页Shanghai Journal of Acupuncture and Moxibustion

基　　金：陕西省陕西省自然科学基础研究计划面上项目(2017JM8108);陕西省自然科学基础研究计划(2020JM-594);国家自然科学基金项目(81674086)。

摘　　要：目的观察电针干预对帕金森病(PD)小鼠大脑黑质组织中酪氨酸羟化酶(TH)、谷胱甘肽过氧化物酶(Gpx-1)以及氧化应激因子超氧化物歧化酶(SOD)蛋白表达的影响,探讨针刺干预改善帕金森病的作用机制。方法采用随机数字表法将40只健康雄性昆明小鼠分为空白组、帕金森模型(PD)组、针刺组、左旋多巴组,每组10只;除空白组外,其余3组均采用MPTP诱导制作小鼠PD模型,造模2周后随机选取两组进行电针及西药干预。干预结束后先采用步长实验和游泳实验对各组小鼠进行行为学评价,再采用Western blot法检测小鼠脑黑质组织SOD-2、Gpx-1和TH表达,免疫组化法检测TH蛋白的表达。结果与空白组比较,PD组小鼠步长距离、游泳时间显著缩短(P<0.05);与PD组比较,针刺组和左旋多巴组小鼠的步长距离和游泳时间加长(P<0.05)。与空白组小鼠比较,PD组小鼠黑质中TH的蛋白量显著减少(P<0.05);与PD组比较,针刺组小鼠黑质中TH蛋白量显著增高(P<0.05),左旋多巴组小鼠黑质中TH蛋白量无明显变化(P>0.05)。与空白组比较,PD组小鼠脑黑质中SOD-2和Gpx-1的表达均显著降低(P<0.05);与PD组比较,针刺组和左旋多巴组SOD-2和Gpx-1的表达水平显著升高(P<0.05)。结论电针干预能提高帕金森模型小鼠的运动功能,其治疗机制可能是通过降低脑黑质中氧化应激蛋白表达实现。Objective To observe the effects of electroacupuncture intervention on the protein expression of tyrosine hydroxylase(TH),glutathione peroxidase(Gpx-1),and oxidative stress factor superoxide dismutase(SOD)in the substantia nigra tissue of Parkinson’s disease(PD)mice.Method Forty healthy male Kunming mice were divided using the random number table method into a blank group,a PD group,an acupuncture group,and a Levodopa group,with 10 mice in each group.Except for the blank group,the other three groups received 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)to induce PD mouse models.Two weeks after the modeling,two groups were randomly selected to receive electroacupuncture and Western medication intervention,respectively.After the intervention,step length and swimming tests were adopted for behavioral assessment for each group.Western blot was used to detect the expression of SOD-2,Gpx-1,and TH in the substantia nigra of mice,and immunohistochemistry was used to detect the protein expression of TH.Result Compared with the blank group,the step length and swimming duration were significantly reduced in the PD group(P<0.05).Compared with the PD group,the step length and swimming duration were increased in the acupuncture and Levodopa groups(P<0.05).Compared with the blank group,the protein expression of TH in mice’s substantia nigra was significantly reduced in the PD group(P<0.05).Compared with the PD group,the TH protein expression in mice’s substantia nigra was markedly increased in the acupuncture group(P<0.05),but the difference in the Levodopa group was statistically insignificant(P>0.05).Compared with the blank group,the expression levels of SOD-2 and Gpx-1 dropped notably in the substantia nigra of mice in the PD group(P<0.05).Compared with the PD group,the expression levels of SOD-2 and Gpx-1 increased significantly in the substantia nigra of mice in both acupuncture and Levodopa groups(P<0.05).Conclusion Electroacupuncture intervention can enhance the motor function of PD model mice,and the treatmen

关 键 词：电针 帕金森病 酪氨酸羟化酶 谷胱甘肽过氧化物酶 超氧化物歧化酶 小鼠 

分 类 号：R2-03[医药卫生—中医学]