不同外源试剂对菜用大豆低温胁迫的调控效应  

Regulatory Effects of Different Exogenous Reagent on Vegetable Soybean Under Low Temperature Stress

作　　者：陈凤琼 陈秋森 刘汉林 褚霈宇 任春元[1] 于高波[1] 王孟雪[1] 张玉先[1] CHEN Feng-qiong;CHEN Qiu-sen;LIU Han-lin;CHU Pei-yu;REN Chun-yuan;YU Gao-bo;WANG Meng-xue;ZHANG Yu-xian(Agricultural College,Heilongjiang Bayi Agricultural University,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学农学院,黑龙江大庆163319

出　　处：《大豆科学》2022年第2期165-171,共7页Soybean Science

基　　金：国家重点研发计划(2018YFD02010000);财政部和农业部中国农业研究系统(CARS-04-01A);黑龙江省博士后科研启动项目(LBH-Q20052);人才引进研究启动计划(XYB202011);黑龙江省农垦局重点科研项目(HKKY190206-1)。

摘　　要：为了筛选可以有效缓解菜用大豆低温胁迫伤害的外源试剂,并明确其对菜用大豆低温胁迫的调控效应。本试验以菜用大豆品种辽鲜1号为供试品种,以赤霉素、乙烯利、阿魏酸、γ-氨基丁酸、壳聚糖和绿原酸为供试试剂,比较外源试剂对低温胁迫下菜用大豆的抗氧化系统、渗透调节物质和氮代谢的影响,筛选能够增强菜用大豆低温胁迫耐性的外源试剂。结果表明:外源GA_(3)、GABA处理显著提高了低温胁迫下菜用大豆的抗氧化酶活性,尤其是SOD和CAT活性,并显著提高了AsA和GSH含量,显著降低了超氧阴离子(O^(-)_(2))含量,缓解了菜用大豆受到的氧化损伤。此外,外源GA_(3)、GABA处理使菜用大豆可溶性糖和蔗糖含量显著增加,增强其细胞保水能力;同时显著提高了菜用大豆氮代谢酶活性,尤其是硝酸还原酶(NR)、谷氨酸合酶(GOGAT)和谷氨酸脱氢酶(GDH)活性,促进菜用大豆氮代谢作用,增强其低温胁迫耐性。由此可知,外源GA_(3)和GABA处理通过调节抗氧化性、渗透调节物质和氮代谢来缓解菜用大豆的耐冷能力。In order to select the exogenous reagents that can effectively alleviate the injury of vegetable soybean,and clarify its regulatory effect on vegetable soybean under low temperature stress,the vegetable soybean variety Liaoxian 1 was used as the test variety,and compared the effects of gibberellin,ethephon,ferulic acidγ-aminobutyric acid,chitosan and chlorogenic acid on antioxidant system,osmoregulation substances and nitrogen metabolism of vegetable soybean under low temperature stress.The results showed that exogenous GA_(3) and GABA treatment significantly increased the antioxidant enzyme activities,especially the activities of SOD and CAT,significantly increased the contents of ASA and GSH,significantly reduced the content of superoxide anion(O^(-)_(2)) and alleviated the oxidative damage of vegetable soybean under low temperature stress.In addition,exogenous GA_(3) and GABA treatment significantly increased the soluble sugar and sucrose content of vegetable soybean and enhanced its cell water holding capacity.At the same time,it significantly improved the activities of nitrogen metabolism enzymes in vegetable soybean,especially the activities of nitrate reductase(NR),glutamate synthase(GOGAT)and glutamate dehydrogenase(GDH),promoted the nitrogen metabolism of vegetable soybean and enhanced the tolerance to low temperature stress.It can be seen that exogenous GA_(3) and GABA treatment can alleviate the cold tolerance of vegetable soybean by regulating antioxidant,osmotic regulatory substances and nitrogen metabolism.

关 键 词：菜用大豆 低温胁迫 外源试剂 赤霉素 Γ-氨基丁酸 

分 类 号：S643.7[农业科学—蔬菜学]