利用MAS选育抗大豆花叶病毒病SC7新品种阜豆169  

Breeding of the New Soybean Cultivar Fudou 169 Resistant to Soybean Mosaic Virus SC7 by Molecular Marker-Assisted Selection

作　　者：王传之 李智 王敏 樊志明 周洪利 杜霄力 孙云飞 于伟 WANG Chuan-zhi;LI Zhi;WANG Min;FAN Zhi-ming;ZHOU Hong-li;DU Xiao-li;SUN Yun-fei;YU Wei(Fuyang Academy of Agricultural Sciences/Fuyang Comprehensive Test Station of National Soybean Industrial Technology System/Anhui Branch of National Soybean Improvement Center,Fuyang 236065,China)

机构地区：[1]阜阳市农业科学院/国家大豆改良中心(安徽)/现代农业产业技术体系阜阳大豆综合试验站,安徽阜阳236065

出　　处：《大豆科学》2022年第2期244-248,共5页Soybean Science

基　　金：国家重点研发计划(2017YFD0101404);国家大豆产业技术体系(CARS04)。

摘　　要：为培育黄淮海和长江流域大豆产区主要大豆花叶病毒流行株系SC7的抗性大豆品种,阜阳市农业科学院/国家大豆改良中心安徽分中心以郑97196为母本、科丰29为父本进行杂交,结合MAS方法选育出抗花叶病毒SC7株系夏大豆新品种阜豆169。两年试验平均粗蛋白含量40.96%,平均粗脂肪含量19.4%。抗性基因连锁分子标记BARCSOYSSR_02_0631鉴定和人工接种鉴定结果均表明,该品种对大豆花叶病毒SC3和SC7株系均表现抗病。对8个大豆疫霉菌株均表现抗病。2019年生产试验产量2668.05 kg·hm^(-2),较对照中黄13增产9.43%。苗期耐渍,后期耐旱,抗倒抗病,落叶性好,不裂荚,高产稳产。于2020年通过安徽省农作物品种审定委员会审定,审定编号为皖审豆20200001。此次MAS技术的应用实现了大豆品系抗花叶病毒SC7相关位点的早期子代选择鉴定,不仅突破了时空条件的限制,而且避免了传统方法接种鉴定时传播花叶病毒的情况,减少了后续工作量,缩短抗病高产大豆品种育种周期。In order to cultivate the soybean varieties resistant to soybean mosaic virus SC7 strain,which are the main soybean mosaic virus strains in the soybean producing areas of the Huang-huai-hai and Yangtze River basins,the Fuyang Academy of Agricultural Sciences/Anhui Branch of the National Soybean Improvement Center used Zheng 97196 as the female parent and Kefeng 29 as the male parent,a new summer soybean variety Fudou 169 with resistance to mosaic virus SC7 was bred by hybridization and MAS(molecular marker-assisted selection).The average crude protein content of the two-year trial was 40.96%,and the average crude fat content was 19.4%.The identification results of resistance gene-linked molecular marker BARCSOYSSR_02_0631 and artificial inoculation showed that the variety showed resistance to soybean mosaic virus SC3 and SC7 strains.Fudou 169 showed disease resistance to 8 isolates of Phytophthora sojae.The production test yield in 2019 was 2668.05 kg·ha^(-1),and an increase of 9.43%compared with the Zhonghuang 13.In 2020,it was approved by the Anhui Provincial Crop Variety Approval Committee,and the approval number was Wanshendou 20200001.The application of MAS technology has realized the early progeny selection and identification of resistant to mosaic virus SC7 in soybean lines,which not only breaks through the limitations of time and space conditions,but also avoids the transmission of mosaic virus in traditional methods of inoculation and identification,reducing the risk of infection,cuts down on the follow-up and shortens the breeding cycle of disease-resistant and high-yield soybean varieties.

关 键 词：大豆 抗花叶病毒病 分子标记辅助选择 阜豆169 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]