肺炎克雷伯氏菌环境菌株血清型及对玉米的致病性测定  

Serotype of Klebsiella pneumoniae Environmental Strain and Its Pathogenicity to Maize

作　　者：原小迪 李钰 陈艳芳 李晶 邵婷 王婷 虞林峰 黄敏 YUAN Xiaodi;LI Yu;CHEN Yanfang;LI Jing;SHAO Ting;WANG Ting;YU Linfeng;HUANG Min(College of Agronomy and Life Sciences,Kunming University,Engineering Research Center for Urban Modern Agriculture of Higher Education in Yunnan Province,Kunming 650214,China)

机构地区：[1]昆明学院农学与生命科学学院,云南省高校都市型现代农业工程研究中心,云南昆明650214

出　　处：《云南农业大学学报（自然科学版）》2022年第3期422-428,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省教育厅科学研究基金项目(2020Y0472);云南省大学生创新创业项目(202011393003);云南省地方本科高校(部分)基础研究联合专项(2018FH001-036)。

摘　　要：【目的】检测肺炎克雷伯氏菌(Klebsiella pneumoniae,KP)环境菌株对玉米的致病性及其与医院常见血清型的关联性。【方法】从环境中取地表水和土壤样品,利用克雷伯氏菌分离培养基(麦康凯肌醇阿东醇羧苄青霉素培养基,MIAC)获得克雷伯氏菌属菌株;通过革兰氏染色、形态学观察、生理生化鉴定和16S rRNA基因序列分析进一步鉴定样品中的KP菌株;以PCR扩增医院常见KP血清型基因条带;用常规接种法检测分离到的KP环境菌株对玉米的致病性。【结果】从栽培草莓的土壤表面分离到1株KP环境菌株E23。该菌株不属于医院常见血清型,但在人工接种条件下能引起玉米顶腐症状。【结论】不同来源的肺炎克雷伯氏菌具有导致易感寄主发病的能力,这一结果可为其跨界侵染机制的研究提供基础。[Purpose]To detect the pathogenicity of Klebsiella pneumoniae(KP)environmental strain on maize and its correlation with common hospital serotypes.[Methods]Klebsiella genus bacteria were isolated from surface water and soil samples from the environment through Klebsiella separation medium(MacConkey inositol adonitol carbenicillin agar,MIAC).KP strain was further identified by Gram staining,morphology observation,physiological and biochemical identification,and 16S rRNA gene sequence analysis.The serotype gene bands of common KP in hospital were amplified by PCR.The pathogenicity of the environmental KP strain to maize was detected by routine inoculation.[Result]K.pneumoniae E23 isolated from the soil surface of strawberry was not a common hospital serotype,but could cause the typical symptoms of maize top rot under artificial inocula-tion.[Conclusion]K.pneumoniae from different sources has the ability to cause the disease of sus-ceptible hosts.It lays the foundation for the study of the mechanism of cross-kingdom infection of K.pneumoniae.

关 键 词：肺炎克雷伯氏菌环境菌株 玉米细菌性顶腐病 血清型 致病性 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]