沙棘总黄酮干预兔耳增生性瘢痕组织块的消退  

Total flavonoids of Hippophae rhamnoides L.interfere with the regression of hypertrophic scar tissue blocks in a rabbit ear model

作　　者：牛梓晗 余扬[1] 艾江 卜盼盼 李文博 苏日耶·热合曼 马少林[1] Niu Zihan;Yu Yang;Ai Jiang;Bu Panpan;Li Wenbo;Suriye·Reheman;Ma Shaolin(Department of Plastic Surgery,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]新疆医科大学第一附属医院整形外科,新疆维吾尔自治区乌鲁木齐市830054

出　　处：《中国组织工程研究》2023年第2期258-263,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81750345),项目负责人:马少林。

摘　　要：背景:沙棘总黄酮可抑制肾脏、肝脏、心肌纤维化,但其对增生性瘢痕纤维化的相关疗效鲜有报道。目的:对兔耳增生性瘢痕组织块局部注射中草药沙棘总黄酮,观察其对增生性瘢痕组织块消退的影响并探讨其作用机制。方法:选择8只新西兰大白兔,每只耳沿腹侧中线两侧各建立3个直径8 mm圆形创面,共96个创面。21 d创面上皮化后,随机分为5组:0.5,1.0,2.0 g/L沙棘总黄酮组,2只/组;二甲基亚砜组(药物溶剂对照)和空白对照组,1只/组。于组织块基底部注射相应药物,每间隔3 d注射1次,连续干预4周。通过苏木精-伊红染色和Masson染色对比各组增生性瘢痕组织块病理组织变化;实时荧光定量PCR检测各组组织块中Ⅰ、Ⅲ型胶原的mRNA表达;Western blot检测各组兔耳瘢痕组织块Ⅰ、Ⅲ型胶原、转化生长因子β1、α-平滑肌肌动蛋白、血管内皮生长因子的蛋白表达。结果与结论:①大体观察:不同质量浓度的沙棘总黄酮局部注射后瘢痕组织块明显软化、变平;②空白对照组大量炎性细胞浸润、血管生成、胶原纤维不规则排列;与空白对照组相比,各沙棘总黄酮组可见整齐的束状胶原纤维分布,新生血管较少,特别是2 g/L沙棘总黄酮组改善较为明显;不同质量浓度沙棘总黄酮组间瘢痕增生指数和胶原密度均低于空白对照组和二甲基亚砜组(P<0.05);③各沙棘总黄酮组和二甲基亚砜组瘢痕组织块的Ⅰ、Ⅲ型胶原mRNA表达水平均显著低于空白对照组,其中2 g/L沙棘总黄酮组的抑制作用最明显(P<0.05);④与空白对照组相比,二甲基亚砜一定程度上可以降低组织块中Ⅲ型胶原、转化生长因子β1、α-平滑肌肌动蛋白、血管内皮生长因子蛋白表达水平;但不同质量浓度沙棘总黄酮组均可明显抑制兔耳增生性瘢痕组织块Ⅰ、Ⅲ型胶原、转化生长因子β1、α-平滑肌肌动蛋白、血管内皮生长因子蛋白的表达水平,且BACKGROUND:Total flavonoids of Hippophae rhamnoides L.can inhibit fibrosis in the kidney,liver and myocardium.However,related studies on hypertrophic scar fibrosis are rarely reported.OBJECTIVE:To observe the effect of total flavonoids of Hippophae rhamnoides L.on the regression of hypertrophic scar in a rabbit ear model and to explore its mechanism.METHODS:Eight New Zealand white rabbits were selected for the study.Three round wounds with a diameter of 8 mm were made on both sides of each ear along the ventral midline,a total of 96 wounds.After 21 days of wound epithelization,all rabbits were randomly divided into five groups:0.5,1.0,2.0 g/L total flavonoids of Hippophae rhamnoides L.groups,two rabbits in each group,and dimethyl sulfoxide group(drug solvent control)and blank control group,one rabbit in each group.Corresponding drugs were injected into the base of tissue once every 3 days for 4 continuous weeks.The pathological changes of hypertrophic scar tissue blocks were observed and compared by hematoxylin-eosin staining and Masson staining.The expressions of type I and III collagen mRNAs in rabbit ear scar tissue were detected by real-time PCR.Western blot was used to detect the expression of type I and III collagen,transforming growth factorβ1,α-smooth muscle actin,and vascular endothelial growth factor proteins in rabbit ear scar tissue blocks.RESULTS AND CONCLUSION:General observation:The scar tissue was softened and flattened significantly after the injection of different concentrations of total flavonoids of Hippophae rhamnoides L.In the blank control group,there were a large number of infiltrated inflammatory cells,angiogenesis and irregular arrangement of collagen fibers.Compared with the blank control group,the neat distribution and loose arrangement of fascicular collagen fibers could be observed in different concentration treatment groups,especially in the 2 g/L group.Scar elevation index and collagen fiber area density in different concentration treatment groups were significantly lower than th

关 键 词：沙棘总黄酮 增生性瘢痕 胶原纤维 转化生长因子Β1 血管内皮生长因子缩略语:转化生长因子β1:transforming growth factor-β1 TGF-β1 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]