有氧和抗阻运动干预2型糖尿病模型大鼠海马抗氧化应激指标和脑源性神经营养因子表达的变化  

Effects of aerobic and resistance exercise on antioxidant stress index and brain-derived neurotrophic factor expression in the hippocampus of type 2 diabetic rats

作　　者：税晓平[1,2,3] 李春莹 李明娟[1] 李顺昌 孙君志[3] 苏全生[3] Shui Xiaoping;Li Chunying;Li Mingjuan;Li Shunchang;Sun Junzhi;Su Quansheng(Sichaun College of Traditional Chinese Medicine,Mianyang 621000,Sichuan Province,China;Mianyang Osteological Hospital,Mianyang 621000,Sichuan Province,China;School of Sports Medicine and Health,Chengdu Sport University,Chengdu 610041,Sichuan Province,China;Mianyang Hospital of Traditional Chinese Medicine,Mianyang 621000,Sichuan Province,China)

机构地区：[1]四川中医药高等专科学校,四川省绵阳市621000 [2]绵阳市骨科医院,四川省绵阳市621000 [3]成都体育学院运动医学与健康学院,四川省成都市610041 [4]绵阳市中医医院,四川省绵阳市621000

出　　处：《中国组织工程研究》2023年第2期264-269,共6页Chinese Journal of Tissue Engineering Research

基　　金：2018年度四川中医药高等专科学校科研项目(18ZRYB03),项目负责人:税晓平;绵阳市高等教育“三三工程”(市级特色专业-康复治疗技术专业建设)项目(202055),项目负责人:税晓平。

摘　　要：背景:糖尿病将导致大脑海马损伤并诱发认知功能障碍,其中氧化应激和神经营养因子表达减少是糖尿病致海马损伤的主要机制。长期规律运动对糖尿病海马损伤有积极干预作用,但相关机制仍需进一步研究。目的:观察有氧和抗阻运动对2型糖尿病大鼠海马抗氧化应激指标热休克蛋白70、核转录因子E2相关因子2、血红素氧合酶1以及脑源性神经营养因子表达的影响。方法:高脂高糖饲料喂养8周后,一次性腹腔注射链脲佐菌素制备2型糖尿病模型大鼠;造模结束后,2型糖尿病大鼠随机分为糖尿病安静对照组、糖尿病有氧运动组和糖尿病抗阻运动组(n=7)。对照组大鼠随机分为安静对照组、有氧运动组、抗阻运动组(n=7)。对照组各组继续普通饲料喂养,糖尿病各组继续高脂高糖饲料喂养。各运动组分别进行8周有氧或抗阻运动干预,8周运动干预结束后,检测各组大鼠空腹血糖和胰岛素抵抗指数;苏木精-伊红染色观察大脑海马结构变化;检测大鼠脑组织氧化应激指标丙二醛水平和超氧化物歧化酶活性;Western blot检测海马热休克蛋白70、核转录因子E2相关因子2、血红素氧合酶1和脑源性神经营养因子蛋白的表达水平。结果与结论:①8周运动干预结束后,与安静对照组比较,糖尿病各组大鼠空腹血糖和胰岛素抵抗指数显著升高(P<0.01);大鼠海马表现出锥体细胞层数减少、细胞空化、细胞坏死和核仁消失等病理改变;与糖尿病安静组比较,2个糖尿病运动干预组空腹血糖和胰岛素抵抗指数均显著下降(P<0.01,P<0.05),大鼠海马细胞空化、坏死和核仁消失等病变程度减轻;②与安静对照组比较,糖尿病各组大鼠大脑中丙二醛水平明显升高(P<0.01),糖尿病安静组超氧化物歧化酶活性明显降低(P<0.01);与糖尿病安静组比较,2个糖尿病运动干预组丙二醛水平明显降低(P<0.01),超氧化物歧化酶活性明显�BACKGROUND:Diabetes mellitus will cause damage to the hippocampus and induce cognitive dysfunction.Oxidative stress and reduced neurotrophic factor expression are the main mechanism of diabetes-induced hippocampal damage.Long-term regular exercise is a positive intervention for diabetes-induced hippocampal damage;however,the related mechanism still needs further research.OBJECTIVE:To observe the effects of aerobic and resistance exercise on the expression of heat shock protein 70,nuclear factor erythroid 2 p45-related factor 2,heme oxygenase 1,and brain-derived neurotrophic factor in the hippocampus of type 2 diabetic rats.METHODS:The rats that had been fed with high-fat and high-sugar diet for 8 weeks were given a single intraperitoneal injection of streptozotocin to prepare a model of type 2 diabetes.Type 2 diabetic rats were randomly divided into diabetic control group,diabetic aerobic exercise group and diabetic resistance exercise group,with seven rats in each group.Another control rats were randomly divided into quiet control group,aerobic exercise group,and resistance exercise group,with seven rats in each group.The control rats continued to be fed with ordinary feed,while the diabetic rats continued to be fed with highfat and high-sugar diet.The aerobic and resistance exercise lasted for 8 weeks in each exercise group.After 8-week exercises,fasting blood glucose and insulin resistance index were measured.Hematoxylin-eosin staining was used to observe the changes of hippocampal structure.The level of malondialdehyde and the activity of superoxide dismutase in brain were measured.The expression of heat shock protein 70,nuclear factor erythroid 2 p45-related factor 2,heme oxygenase 1,and brain-derived neurotrophic factor in the hippocampus were detected by western blot assay.RESULTS AND CONCLUSION:After 8 weeks of exercise,compared with the quiet control group,fasting blood glucose and insulin resistance index of all diabetic rats were significantly increased(P<0.01).The hippocampus of all diabetic rats show

关 键 词：运动 糖尿病 海马 氧化应激 脑源性神经营养因子 大鼠 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]