核因子κB受体活化因子信号转导机制与破骨细胞的活化  

Receptor activator of nuclear factor-kappa B ligand signal transduction mechanism and osteoclast activation

作　　者：陈锋[1] 任国武 章晓云[2] 陈跃平[2] 石儒生 Chen Feng;Ren Guowu;Zhang Xiaoyun;Chen Yueping;Shi Rusheng(Guangxi University of Chinese Medicine,Nanning 530000,Guangxi Zhuang Autonomous Region,China;Department of Orthopedics,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530011,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]广西中医药大学,广西壮族自治区南宁市530000 [2]广西中医药大学附属瑞康医院骨科,广西壮族自治区南宁市530011

出　　处：《中国组织工程研究》2023年第2期293-299,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81960803,81760796),项目负责人:陈跃平;广西自然科学基金青年基金(2020GXNSFBA159053),项目负责人:章晓云;广西高校青年教师基础能力提升项目(2019KY0352),项目负责人:章晓云;金天格中青年基金项目(项目61),项目负责人:章晓云。

摘　　要：背景:破骨细胞是目前已知的唯一一种骨吸收细胞,其生命活动对骨骼的正常发育和骨骼损伤修复至关重要。在绝大多数骨病中,破骨细胞均显示出异常增殖分化和骨吸收活性增加,而核因子κB受体活化因子信号是调控破骨细胞生命过程的关键信号通路。目的:总结对破骨细胞核因子κB受体活化因子信号下游靶点及DNA转录因子的最新研究进展,为相关疾病的研究和治疗提供依据。方法:文献检索数据库包括中国知网、万方、维普数据库、PubMed、Embase及Web of Science数据库,中文检索词为“破骨细胞,破骨前体细胞,骨质疏松症,骨代谢,发病机制,表观遗传学,信号通路,信号传导,转录因子,组织工程”,英文检索词为“osteoclasts,osteoclast precursor cells,osteoporosis,bone metabolism,pathogenesis,epigenetics,signaling pathway,signal transduction,transcription factors,tissue engineering”,时间设置为2017-2021年,根据纳入和排除标准共筛选52篇文献。结果与结论:核因子κB受体活化因子的特殊结构决定了其信号传导需要与肿瘤坏死因子受体激活因子6结合来募集多种蛋白、活性酶以及细胞因子,形成具有内在酶活性的核因子κB受体活化因子复合物;该复合物通过激活核因子κB、丝裂原活化蛋白激酶等信号通路的传导,最终调控破骨细胞分化、增殖、骨吸收等生命过程。BACKGROUND:Osteoclasts are the only known type of bone resorption cells,and its life activities are essential to the normal development of bones and the repair of bone damage.Osteoclasts show abnormal proliferation and differentiation and increased bone resorption activity in most bone diseases,while receptor activator of nuclear factor-kappa B(RANK)is a key signal pathway that regulates osteoclastogenesis.OBJECTIVE:To summarize the latest research progress on the downstream targets of osteoclast RANK signal and DNA transcription factors at home and abroad,and provide a basis for the research and treatment of related diseases.METHODS:A computer-based retrieval was conducted in CNKI,WanFang,VIP,PubMed,Embase and Web of Science databases.The search terms were“osteoclasts,osteoclast precursor cells,osteoporosis,bone metabolism,pathogenesis,epigenetics,signaling pathway,signal transduction,transcription factors,tissue engineering”in Chinese and English.The retrieval time was set to 2017-2021.Finally 52 articles were included according to the inclusion and exclusion criteria.RESULTS AND CONCLUSION:The special structure of RANK determines that its signal transduction needs to be combined with tumor necrosis factor receptor-associated factor 6 to recruit a variety of proteins,active enzymes and cytokines to form a RANK complex with intrinsic enzyme activity.This complex then activates nuclear factor-κB,mitogen-activated protein kinase and other signal pathways,ultimately regulating osteoclast differentiation,proliferation,and bone resorption.

关 键 词：破骨细胞 核因子ΚB受体活化因子 细胞信号通路 骨质疏松症 骨组织工程 综述 

分 类 号：R459.9[医药卫生—治疗学] R329[医药卫生—临床医学]