精子活性氧类物质与其形态及DNA碎片的相关性  

The correlation between the level of oxygen active substances of sperm and their morphology and DNA fragments

作　　者：宋晓庆 李采霞 潘小红 杨春燕[1] 彭晓旭 张艳茹[1] 高文怡 邓云[1] SONG Xiaoqing;LI Caixia;PAN Xiaohong;YANG Chunyan;PENG Xiaoxu;ZHANG Yanru;GAO Wenyi;DENG Yun(Joint Logistic Support Force 904 Hospital,Wuxi,Jiangsu Province,214000)

机构地区：[1]中国人民解放军联勤保障部队第904医院,无锡214000

出　　处：《中国计划生育学杂志》2022年第2期387-390,共4页Chinese Journal of Family Planning

摘　　要：目的:探究精子活性氧类物质(ROS)与精子形态及精子DNA损伤的相关性,为男性不孕症抗氧化治疗提供理论依据。方法:选择2018年8月-2020年8月本院就诊的男性不育患者精液标本250例,检测精子形态学、精子DNA完整性以及精子ROS,根据精子形态分为正常形态、头部畸形、颈部畸形、尾部畸形以及混合畸形组,根据精子DNA碎片指数(DFI)分为<15%、15%~30%、>30%组,分析不同组中精子ROS水平及其相关性。结果:250例患者中精子形态正常占60.4%,异常39.6%(头部畸形47.5%、颈部畸形35.4%、尾部畸形13.1%、混合畸形4.0%),各畸形组间ROS水平(715.49±203.82 U/L、711.39±206.24 U/L、718.83±201.57 U/L、721.31±200.67 U/L)无差异但均高于正常组(462.76±148.76 U/L)(P<0.05);精子DFI<15%(55.6%)、DFI 15%~30%(32.4%)、DFI>30%(12.0%)有差异,ROS水平DFI>30%组(719.74±205.39 U/L)、DFI 15~30%组(637.24±183.65 U/L)、DFI<15%组(457.68±151.46 U/L)依次降低(均P<0.05)。Pearson相关性分析,精子形态正常率与精子ROS水平存在负相关,颈部畸形、颈部畸形、尾部畸形均与精子ROS水平存在正相关,且以头部畸形相关性最高,精子DFI值与精子ROS水平存在正相关性(均P<0.05)。结论:异常形态的精子可能是产生氧自由基的重要来源,而精子DNA碎片增多会提高精子ROS水平,可能加速生殖细胞凋亡过程,导致男性不育。Objective:To explore the correlation between the level of sperm oxygen active substances(OAS)of the men with infertility and their sperm morphological morphology and sperm DNA fragments,so as to provide theoretical basis for the antioxidation treatment of these men.Methods:250 male semen samples from August 2018 to August 2020 were selected to detect their sperm morphology,sperm DNA integrity,and the level of OAS.According to sperm morphology,these sperms were divided into group A1(sperm with normal morphology),group A2(sperm with head deformity,group A3(sperm with neck deformity,group A4(sperm with tail deformity),and group A5(sperm with mixed deformity).These sperms were also divided into group B1(sperm with sperm DNA fragment index(DFI)<15%),group B2(sperm with sperm DFI 15%-30%,and group B3(sperm with sperm DFI>30%)according to the rate of DFI.The sperm OAS levels in different groups were analyzed and its correlation with male infertility was analyzed.Results:Among these 250 sperm samples,there were 60.4%samples with normal sperm morphology,and there were 39.6%samples with abnormal sperm morphology,which included sperm head deformity of 47.5%,sperm neck deformity of 35.4%,sperm tail deformity 13.1%,and sperm mixed deformity 4.0%.There were no significant difference in the OAS level of sperm(715.49±203.82 U/L vs.711.39±206.24 U/L vs.718.83±201.57 U/L vs.721.31±200.67 U/L)among group A2,group A3,group A4,and group A5,but the OAS level of sperm in group A2,group A3,group A4,or group A5 was all significantly higher than that(462.76±148.76 U/L)in group A1(P<0.05).The rates of sperm DFI in group B1,group B2,and group B3 were 55.6%,32.4%,and 12.0%,respectively,which had significant different among these groups.The OAS level of sperm in group B3(719.74±205.39 U/L),in group B2(637.24±183.65 U/L),and in group B1(457.68±151.46 U/L)had decreased gradually(all P<0.05).Pearson correlation analysis showed that normal sperm morphology rate of the men was negatively correlated with their sperm OAS level.The rate of sp

关 键 词：男性不育 精子形态 活性氧类物质 DNA碎片 相关性 

分 类 号：R698.2[医药卫生—泌尿科学]