子宫内膜异位组织中MiR-26b与COX-2表达关系及意义  

Relatinship between the expression of MicroRNA-26b of patients with endometriosis and their level of cyclooxygenase-2 and the significances of the expression of MicroRNA-26b and the level of cyclooxygenase-2

作　　者：钱进[1] 朱晓琴[1] 王永霞[1] 马宗丽 QIAN Jin;ZHU Xiaoqin;WANG Yongxia;MA Zongli(Rugao People's Hospital,Nantong,Jiangsu Province,226500)

机构地区：[1]江苏省如皋市人民医院,226500

出　　处：《中国计划生育学杂志》2022年第3期664-667,共4页Chinese Journal of Family Planning

基　　金：南通市市级科技计划(指导性)项目(MSZ19143)。

摘　　要：目的:观察子宫内膜异位症(EMs)患者在位子宫内膜组织中微小RNA26b(MiR-26)和环氧化酶2(COX-2)的表达。方法:收集2015年5月-2019年12月本院确诊EMs患者195例(EMs组),行子宫肌瘤切除术或因宫颈病变接受全子宫切除术无EMs患者50例(对照组)。免疫组化法检测内膜组织COX-2蛋白表达,实时荧光定量检测内膜组织中MiR-26b的表达,比较两组差异,分析EMs组COX-2与MiR-26b相关性,及与EMs分期和疼痛分级关系。结果:EMs组COX-2表达水平高于对照组,MiR-26b mRNA表达(0.81±0.27)低于对照组(1.93±0.46)(P<0.05)。COX-2与疼痛分级正相关(r=0.724),MiR-26b与疼痛分级负相关(r=-0.308)(均P<0.05),r-AFS分期与COX-2和MiR-26b表达无明显相关性。EMs组COX-2与MiR-26b表达呈负相关(r=-0.522)(P<0.05),对照组COX-2与MiR-26b表达无相关性。结论:EMs患者在位内膜组织中COX-2高表达、MiR-26b低表达,二者负相关,MiR-26b可能参与了EMs病理过程中COX-2的调节。Objective: To observe the expression of cyclooxygenase(COX)-2 in endometrial tissue of patients with endometriosis(EMS). Methods: 195 patients with EMS were selected in study group, and 50 patients without EMS underwent myomectomy or hysterectomy due to cervical lesions were selected in control group from May 2015 to December 2019. The expression of COX-2 protein of these patients was detected by immunohistochemistry and MiR-26 b expression in endometrial tissue of these patients was detected by real-time PCR, and the difference of which of the patients were compared between the two groups. The relationship between the COX-2 level of the patients in the study group and their mir-26 b expression was analyzed. The relationship between the COX-2 level and the expression of the mir-26 b of the patients in the study group and their EMS stage and pain grade was also analyzed. Results: The expression level of COX-2 of the patients in the study group was significantly higher than that of the patients in the control group, but MiR-26 b mRNA expression(0.81±0.27) of the patients in the study group was significantly lower than that(1.93±0.46) of the patients in the control group(P<0.05). There was a positive correlation between the COX-2 expression of the patients in the study group and their pain grade(r=0.724), and there was a weak negative correlation between the Mir-26b expression and their pain grade(r=-0.308)(all P<0.05). There was no significant correlation between r-AFS stage of Ems of the patients and their expressions of COX-2 and Mir-26b. The expression of COX-2 of the patients in the study group was negatively correlated with their Mir-26b expression(r=-0.522)(P<0.05). There was no correlation between the COX-2 expression of the patients in the control group and their Mir-26b expression. Conclusion: The expression level of COX-2 in endometrial tissue of the patients with Ems is high, but the expression of MiR-26 b is low, and the expression level of COX-2 is negative correlation with the expression of MiR-26 b

关 键 词：子宫内膜异位症 在位内膜组织 环氧化酶-2:微小RNA-26b 相关性 

分 类 号：R711.71[医药卫生—妇产科学]