野生大豆染色体片段代换系群体中与百粒重关联的野生片段及其候选基因  

Wild segments associated with 100-seed weight and their candidate genes in a wild chromosome segment substitution line population

作　　者：刘成 张雅轩 陈先连 韩伟[1] 邢光南[1] 贺建波[1] 张焦平 张逢凯 孙磊[1] 李宁[1] 王吴彬[1] 盖钧镒[1] LIU Cheng;ZHANG Ya-Xuan;CHEN Xian-Lian;HAN Wei;XING Guang-Nan;HE Jian-Bo;ZHANG Jiao-Ping;ZHANG Feng-Kai;SUN Lei;LI Ning;WANG Wu-Bin;GAI Jun-Yi(Soybean Research Institute/National Center for Soybean Improvement/Key Laboratory for Biology and Genetic Improvement of Soybean(General)of Ministry of Agriculture and Rural Affairs/National Key Laboratory of Crop Genetics and Germplasm Enhancement/Jiangsu Collaborative Innovation Center for Modern Crop Production,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China)

机构地区：[1]南京农业大学大豆研究所/国家大豆改良中心/农业农村部大豆生物学与遗传育种重点实验室(综合)/作物遗传与种质创新国家重点实验室/江苏省现代作物生产协同创新中心,江苏南京210095

出　　处：《作物学报》2022年第8期1884-1893,共10页Acta Agronomica Sinica

基　　金：江苏省农业科技自主创新资金项目(CX(20)2007);中央高校基本科研业务费专项(KYZ202103);国家自然科学基金项目(31601325);长江学者和创新团队发展计划项目(PCSIRT_17R55);国家现代农业产业技术体系建设专项(CARS-04)资助。

摘　　要：一年生野生大豆是栽培大豆的祖先,在长期驯化改良过程中,百粒重逐渐增大,阐明该变化的遗传基础,对大豆的进化研究与品种改良具有重要意义。为了解析大豆百粒重驯化的遗传基础,本研究以177份全基因组重测序的野生大豆染色体片段代换系(SojaCSSLP5)为材料,通过3个不同环境的表型评价,检测到13个与大豆百粒重相关的野生染色体片段,均具有减小大豆百粒重的加性效应,变幅为-0.49~-1.19 g,这与野生大豆具有较小百粒重相符。检测到的这些野生染色体片段分布在大豆11条染色体上,可以解释76.70%的表型变异,单个片段表型贡献率变幅为2.45%~15.14%。其中片段Gm03_LDB_15和Gm12_LDB_46的贡献率超过10%,为大豆百粒重由野生向栽培进化的大效应片段。结合双亲栽培大豆南农1138-2和野生大豆N24852的转录组数据和基因组数据,在这些区段内共预测到13个百粒重候选基因,涉及以下调控植物种子大小的途径:泛素蛋白激酶调控途径、G蛋白信号途径、裂原活化蛋白激酶途径、植物激素途径、转录调控因子途径和HAIKU途径。与前人利用栽培大豆的研究结果相比,本研究检测到13个大豆百粒重相关野生染色体片段中有4个片段是新检测到的,表明有9个野生染色体片段可能为野生大豆传递给栽培大豆的共享片段,而这4个新检测到的野生染色体片段相对应栽培片段可能为栽培大豆特有的进化片段。Annual wild soybean is the ancestor of cultivated soybean.The 100-seed weight gradually increases in the long-term domestication process.Clarifying the genetic basis of this change is of great significance to the evolutionary research and variety improvement of soybean.In order to analyze the genetic basis of 100-seed weight during soybean domestication,a wild soybean chromosome segment substitution line population(SojaCSSLP5)composed of 177 whole-genome resequencing lines were used in this study.13 QTLs/segments of 100-seed weight were detected by phenotypic evaluation in three different environments.All of 13 wild chromosome segments had the additive effect of reducing 100-seed weight,ranging from-0.49 g to-1.19 g,which was consistent with the smaller 100-seed weight of wild soybeans.These detected domesticated segments from 11 chromosomes explained 76.70%of the phenotypic variation,and the phenotypic contribution rate of a single segment ranged from 2.45%to 15.14%.The contribution rate of segments Gm03_LDB_15 and Gm12_LDB_46 exceeded 10%,which were major influence on the evolution of 100-seed weight of wild soybeans.Combined the transcriptome data and genome data of parental cultivated soybean Nannong 1138-2 and wild soybean N24852,a total of 13 candidate genes were predicted in these segments,and were involved in the pathways of plant seed size,including ubiquitin protein kinase regulatory pathway,G protein signal pathway,mitogen-activated protein kinase pathway,plant hormone pathway,transcription regulator pathway,and IKU(HAIKU)pathway.Compared with previous QTLs mapping results with cultivated soybeans,4 of the 13 QTLs/segments were newly detected in this study,indicating that 9 wild chromosome segments might be passed to cultivated soybeans during domestication,and the corresponding cultivated segments of these 4 wild segments may be unique evolutionary segments of cultivated soybean.

关 键 词：野生大豆 染色体片段代换系 百粒重 数量性状位点 

分 类 号：S565.1[农业科学—作物学]