小麦顶端小穗退化突变体asd1基因定位  

Gene mapping of apical spikelet degeneration mutant asd1 in wheat

作　　者：杜启迪 郭会君[2] 熊宏春 谢永盾[2] 赵林姝[2] 古佳玉[2] 赵世荣[2] 丁玉萍[2] 宋希云[1] 刘录祥[2] DU Qi-Di;GUO Hui-Jun;XIONG Hong-Chun;XIE Yong-Dun;ZHAO Lin-Shu;GU Jia-Yu;ZHAO Shi-Rong;DING Yu-Ping;SONG Xi-Yun;LIU Lu-Xiang(College of Life Sciences,Qingdao Agricultural University,Qingdao 266109,Shandong,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement/National Center of Space Mutagenesis for Crop Improvement,Beijing 100081,China)

机构地区：[1]青岛农业大学生命科学学院,山东青岛266109 [2]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/国家农作物航天诱变技术改良中心,北京100081

出　　处：《作物学报》2022年第8期1905-1913,共9页Acta Agronomica Sinica

基　　金：国家现代农业产业技术体系建设专项(CARS-03);中国农业科学院科技创新工程重大科研任务(CAAS-ZDRW202002)资助;supported by the China Agriculture Research System(CARS-03)。

摘　　要：穗粒数是小麦产量三要素建成的关键因子,深入挖掘穗部发育调控基因有助于培育高产小麦品种。以小麦品种京411为野生型,经EMS诱变获得了表型稳定的小穗退化突变体asd1(apical spikelet degeneration 1)。该突变体表现顶端小穗明显退化,穗长缩短了约40%,结实小穗数减少了约35%,穗粒数显著减少了54%,同时株高也明显降低。利用京411×asd1遗传群体的F2和F3代表型数据分析表明,顶端小穗退化性状受1对主效隐性基因控制。采用混合群体分离分析法(BSA),结合测序所得SNP位点,在7A染色体上开发了7个KASP标记,将目标突变基因定位在7A染色体短臂9.91 Mb物理区间内,遗传距离为17.62 cM,推断该区段存在一个新的控制小麦花器官发育及穗部形态发育的重要基因。本研究所鉴定的小麦穗发育控制区段有助于深入解析小麦小穗形成的遗传基础,为进一步揭示小麦产量形成的分子机理提供突变基因。The number of grains per spike is one of the three key factors for yield and production in wheat.It is helpful to cultivate high-yield wheat varieties to elaborate the regulation pathway of spike development.A stable mutant of apical spikelet degeneration(asd1)was derived by EMS treatment,the wheat variety Jing 411 was used as wild type.The mutant asd1 showed that the apical spikelets were significantly degenerated,the spike length was shortened by 40%,the number of spikelets per spike was reduced by 35%,the number of grains per spike was significantly reduced by 54%,and the plant height was also significantly reduced.A genetic segregation population,Jing 411×asd1,was developed,and phenotypic performances of both F2 and F3 were investigated in field.It showed that the degeneration of apical spikelet was controlled by a recessive gene.Using Bulked Segregant Analysis(BSA)and exon capture,SNPs were obtained and 7 KASP markers were further developed.The target mutation gene was mapped on chromosome 7A and narrowed down to a 9.91 Mb region on 7AS,which represented a genetic distance of 17.62 cM.The region had not yet been reported in regulating spikelet development and the target mutant gene might be a novel gene that controls the development of spike morphology in wheat,which will bring new insights for further understanding of the genetic basis of spikelet development in wheat.

关 键 词：小麦 顶端小穗退化 遗传分析 基因定位 

分 类 号：S512.1[农业科学—作物学]