中国谷子名米品种遗传多样性与亲缘关系研究  

Genetic diversity and genetic relationship of Chinese traditional foxtail millet accessions

作　　者：王蓉 陈小红 王倩 刘少雄[2] 陆平 刁现民[2] 刘敏轩[2] 王瑞云[1,3] WANG Rong;CHEN Xiao-Hong;WANG Qian;LIU Shao-Xiong;LU Ping;DIAO Xian-Min;LIU Min-Xuan;WANG Rui-Yun(College of Agronomy,Shanxi Agricultural University,Taigu 030801,Shanxi,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Center for Agricultural Genetic Resources Research,Shanxi Agricultural University/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture and Rural Affairs/Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops,Taiyuan 030031,Shanxi,China)

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]中国农业科学院作物科学研究所,北京100081 [3]山西农业大学农业基因资源研究中心/农业农村部黄土高原作物基因资源与种质创制重点实验室/杂粮种质资源发掘与遗传改良山西省重点实验室,山西太原030031

出　　处：《作物学报》2022年第8期1914-1925,共12页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2019YFD1000700,2019YFD1000701);农作物种质资源保护与利用专项(2021NWB036-06);国家现代农业产业技术体系建设专项(CARS-06);中国农业科学院科技创新工程;山西省现代农业产业技术体系建设专项(杂粮)资助。

摘　　要：中国是谷子的起源地,历史上的四大传统名米分别为沁州黄、桃花米、龙山小米和金谷米,由于其具有良好的商品与食用品质特征,曾被选为皇家贡米。为探究谷子四大名米的遗传多样性以及与同名品种间的亲缘关系,本研究利用46对SSR标记和22对SRAP标记分别对四大名米及其同名品种共l79份材料的遗传差异进行分析。SSR结果表明,材料间遗传距离为0.0435~0.6304,平均0.3267,PIC为0.2406,等位基因频率为0.7845,遗传多样性为0.2968;基于UPGMA将179份资源聚为10个群组,聚类与其来源有一定相关性;基于Structure和主成分分析均可将材料分为3个类群。SRAP结果表明材料间遗传距离为0.1818~0.9545,平均为0.6001,PIC为0.5257,等位基因频率为0.5718,遗传多样性为0.5688;基于UPGMA将179份材料分为14个类群;基于Structure和主成分分析均可将材料分为3个类群,类群间界限清楚,类群划分与其来源一致。China is the birthplace of foxtail millet,four traditional foxtail millet accessions of Qinzhouhuang,Taohuami,Longshanxiaomi,and Jingumi because of its good quality characteristics,has been selected as the royal tribute and is loved by people in Chinese history.To explore the genetic diversity of four Chinese famous foxtail millet and the genetic relationships among the namesakes,SSR and SRAP markers were used to analyze the genetic difference of 179 foxtail millet varieties.Results based on SSR markers showed that the genetic distance ranged from 0.0435 to 0.6304 with an average of 0.3267,the PIC was 0.2406,the allele frequency was 0.7845,and the genetic diversity was 0.2968.Based on UPGMA,179 materials were clustered into 10groups,and the clusters had certain correlation with their sources.The materials were divided into three groups based on structure and three groups based on principal component analysis.SRAP marker analysis revealed that the genetic distance ranged from 0.1818 to 0.9545 with an average of 0.6001,and the PIC,the allele frequency,and the genetic diversity were 0.5257,0.5718,and 0.5688,respectively.Based on UPGMA,179 materials were clustered into 14 groups.The materials were divided into three groups based on structure and principal component analysis.The groups were clearly divided and the origin of the groups was consistent with their source.

关 键 词：谷子传统名米 沁州黄 桃花米 SSR SRAP 遗传多样性 

分 类 号：S515[农业科学—作物学]