大豆主要营养品质性状相关分子标记的育种应用潜力评价  

Potential evaluation of molecular markers related to major nutritional quality traits in soybean breeding

作　　者：怀园园 张晟瑞 武婷婷[1] AZAM Muhammad 李静[1] 孙石[1] 韩天富[1] 李斌[1] 孙君明[1] HUAI Yuan-Yuan;ZHANG Sheng-Rui;WU Ting-Ting;AZAM Muhammad;LI Jing;SUN Shi;HAN Tian-Fu;LI Bin;SUN Jun-Ming(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Engineering Research Center of Crop Molecular Breeding/Key Laboratory of Soybean Biology,the Ministry of Agriculture and Rural Affairs,Beijing 100081,China)

机构地区：[1]中国农业科学院作物科学研究所/作物分子育种国家工程研究中心/农业农村部大豆生物学重点实验室,北京100081

出　　处：《作物学报》2022年第8期1957-1976,共20页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2017YFD0101400);中国农业科学院农业科技创新工程项目(2060302-2)资助。

摘　　要：大豆营养品质性状多为数量性状,受多基因调控。目前,已定位到大量与营养品质性状相关的分子标记,但经过大豆育种群体验证的可用标记却很少。本研究以288份黄淮海地区选育的大豆品种和19份野生/半野生大豆种质为材料组成大豆自然群体,利用近红外光谱法、气相色谱法和高效液相色谱法分析其蛋白质、脂肪、脂肪酸和异黄酮组分含量;选用已报道的与营养品质性状紧密连锁的18个SSR标记,采用毛细管电泳方法进行基因型鉴定。采用关联分析方法验证分子标记的选择效果,共检测出与脂肪含量关联的标记3个,与蛋脂总和关联的标记3个,与棕榈酸关联的标记1个,与硬脂酸关联的标记1个,与油酸关联的标记2个,与亚麻酸关联的标记2个,同时发掘出这些位点的优异等位变异,表明上述验证的分子标记可用于大豆营养品质分子育种中。Soybean nutritional quality traits are generally quantitative traits and are regulated by multiple genes.At present,a large number of molecular markers related to quality traits have been located,but there were few available markers verified in soybean breeding populations.In this study,we selected 288 soybean varieties from Huang-Huai-Hai region and 19 wild/semi-wild soybean accessions as a natural population.We analyzed their protein,oil,fatty acid,and isoflavone contents by NIR,GC,and HPLC methods.We selected 18 SSR markers closely linked to quality traits to identify their genotypes by capillary electrophoresis method,and verify the molecular markers associated with nutritional quality traits using association analysis based on the Tassel 2.1 software.The results showed that there were three markers associated with oil content,three markers associated with the content of protein plus oil,one marker associated with palmitic acid,one marker associated with stearic acid,two markers associated with oleic acid,and two markers associated with linolenic acid,and the elite alleles of these loci were also investigated.Our study provided reliable molecular markers for soybean quality molecular breeding.

关 键 词：大豆 品质性状 SSR标记 关联分析 优异等位变异 

分 类 号：S565.1[农业科学—作物学]