抗番茄黄化曲叶病毒病基因ty-5的功能标记开发与利用  

Development and Utilization of Functional Marker for Resisting ty-5 Gene of Tomato Yellow Leaf Curl Disease

作　　者：邹虎成 李玉洪 覃柳兰 庄映红 滕献有 潘玲华 ZOU Hucheng;LI Yuhong;QIN Liulan;ZHUANG Yinghon;TENG Xianyou;PAN Linghua(Guilin City Research Center for Agricultural Sciences,Guilin Branch,Guangxi Academy of Agricultural Sciences,Guilin 541006,Guangxi,China)

机构地区：[1]桂林市农业科学研究中心,广西农业科学院桂林分院,广西桂林541006

出　　处：《中国蔬菜》2022年第5期23-28,共6页China Vegetables

基　　金：国家现代农业产业技术体系广西蔬菜瓜果创新团队项目(nycytxgxcxtd-10);广西科技攻关计划项目(桂科攻1598006-2F);桂林市科学研究与技术开发计划项目(20210119z)。

摘　　要：番茄黄化曲叶病毒病是番茄重要病害之一。在ty-5基因编码序列分析的基础上,设计ty-5基因的功能标记,对番茄黄化曲叶病毒病抗病自交系t33-14、感病自交系t2301、t33-14×t2301的F_(2)株系和不同来源的26份番茄材料进行基因型鉴定,并进行表型相关性分析。结果表明,功能标记TySNP能准确地区分育种亲本以及杂交后代群体的番茄黄化曲叶病毒病抗病材料纯合基因型、感病材料纯合基因型以及感病杂合基因型,且不同的基因型跟表型能较好对应吻合,可对杂交后代进行有效选择。因此,功能标记TySNP可用于番茄分子标记辅助育种,可为定向改良番茄品种对黄化曲叶病毒病的抗性提供分子辅助育种手段。Tomato yellow leaf curl virus disease is one of the important diseases for tomato.On the basis of analyzing ty-5 gene coding sequence,this experiment designed functional marker of ty-5 gene;conducted genotype identification on tomato resistant inbred line‘t33-14’to tomato yellow leaf curl virus(TYLCV),susceptible inbred line t2301 to TYLCV,F_(2) strain of inbred line‘t33-14’בt2301’,and 26 tomato materials from different sources;and carried out phenotype correlation analysis.The results indicated that functional marker TySNP could accurately distinguish breeding parents from homozygous genotype of tomato material resistant to TYLCV,homozygous genotype of tomato susceptible materials and susceptible heterozygous genotype in hybrid progeny populations.Different genotypes could correspond better with phenotypes,and be used for effective selection for hybrid progeny.Therefore,functional marker TySNP could be used in tomato MAS breeding,and provided a marker-assisted breeding means for directional improvement of tomato cultivars resistant to TYLCV.

关 键 词：番茄 黄化曲叶病毒病 功能标记TySNP ty-5 

分 类 号：S436.412.11[农业科学—农业昆虫与害虫防治]