杭州芦笋茎枯病病原鉴定与防治  

Pathogen Identification and Control of Asparagus Stem Blight in Hangzhou City

作　　者：石延霞[1] 王朵 张旭娟 施建军 路雅静 谢学文[1] 柴阿丽[1] 李磊 李宝聚[1] SHI Yanxia;WANG Duo;ZHANG Xujuan;SHI Jianjun;LU Yajing;XIE Xuewen;CHAI Ali;LI Lei;LI Baoju(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Hangzhou Jiahui Agricultural Development Co.,Ltd.,Hangzhou 311200,Zhejiang,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]杭州佳惠农业开发有限公司,浙江杭州311200

出　　处：《中国蔬菜》2022年第5期37-43,共7页China Vegetables

基　　金：国家大宗蔬菜产业技术体系项目(CARS-23),中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS),特色功能蔬菜种质创新与新品种选育项目(2016C02051-8-2)。

摘　　要：2018—2020年,杭州佳慧芦笋种植基地疑似发现芦笋茎枯病危害,为了明确病原菌种类,将采集的病样进行致病菌分离纯化和鉴定,明确该病害为芦笋茎枯病,根据病原菌形态特征、通用引物及特异性引物扩增序列比对结果,该病原菌与天门冬拟茎点霉〔Phomopsis asparagi(Sacc.)Bubak〕同源性为100%,鉴定为天门冬拟茎点霉。生物学活性测定结果表明,25%吡唑醚菌酯悬浮剂对该病菌菌丝完全抑制,75%百菌清可湿性粉剂对菌丝的抑制作用次之,EC_(50)为0.13μg·mL^(-1)。盆栽试验验证,25%吡唑醚菌酯悬浮剂对茎枯病防治效果最好,达94.56%,可作为防治芦笋茎枯病高效药剂。During 2018—2020,suspected asparagus stem blight case was discovered in Hangzhou Jiahui asparagus planting base.In order to definit the pathogenic species,this study conducted seperation,purification and identification on collected disease samples.It was definited that the disease was asparagus stem blight.According to pathogenic bacteria morphology character,and comparison results of amplification sequences between universal primer and specific primers,the homology of this pathogenic bacteria and Phomopsis asparagi(Sacc.)Bubak was 100%.It was identified as rhizome mildew of asparagine.The results of biological activity determination indicated that mycelium of this pathogenic bacteria was completely inhibited by 25% pyraclostrobin suspending agent.The inhibiting effect to this pathogenic bacteria was followed by 75% chlorothalonil wettable powder.EC_(50) was 0.13μg·mL^(-1).Pot experiment verified that 25% pyraclostrobin suspending agent had the best effect on controlling stem blight,reaching 94.56%.It could be used as a highly effective agent for preventing and controlling asparagus stem blight.

关 键 词：芦笋茎枯病 天门冬拟茎点霉 生物活性测定 病原菌鉴定 吡唑醚菌酯 

分 类 号：S436.44[农业科学—农业昆虫与害虫防治]