黑龙江省马铃薯疮痂病菌种群结构及PAI致病基因分析  

Analysis of Potato Common Scab Population Structure and PAI Pathogenic Gene in Heilongjiang Province

作　　者：张铉哲[1] 赵雪[1] 陈苏慧 徐浩然 任雪琦 朱二龙 ZHANG Xuanzhe;ZHAO Xue;CHEN Suhui;XU Haoran;REN Xueqi;ZHU Erlong(College of Agriculture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China)

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030

出　　处：《中国蔬菜》2022年第5期53-59,共7页China Vegetables

基　　金：黑龙江省马铃薯现代农业产业技术协同创新体系项目(HNWJZT201801)。

摘　　要：2018—2020年,从黑龙江省6个市(哈尔滨市、佳木斯市、鹤岗市、绥化市、牡丹江市和鸡西市)采集马铃薯疮痂病薯块,采用稀释分离法对马铃薯样品进行病原菌分离,利用萝卜幼苗法进行分离菌株致病性测定。根据形态特征、生理生化特性,结合16S rDNA序列对疮痂病菌进行分类鉴定,并进一步分析了致病菌的PAI致病基因组成。结果表明,从6个市采集到的154份马铃薯样品中分离鉴定获得263株链霉菌致病菌株,共有5种,为S.scabies、S.acidiscabies、S.bottropensis、S.rochei和S.lavendulae。其中S.scabies是优势菌株,S.bottropensis、S.rochei和S.lavendulae是黑龙江省首次报道的马铃薯疮痂病菌。通过对致病链霉菌进行PAI致病基因(txtAB、tomA、nec1)的PCR扩增,共检测出2种致病基因类型txtAB^(+)/tomA^(+)/nec1^(+)和txtAB^(-)/tomA^(+)/nec1^(+)。This study collected potato tubers infected with potato common scab from 6 cities of Heilongjiang Province(Harbin,Jiamusi,Hegang,Suihua,Mudanjiang and Jixi)from 2018-2020;conducted pathogen isolation on potato samples by dilution method;and tested the pathogenicity of the isolated Streptomyces spp.strains by radish seedling method.This study also carried out classification and identification on pathogenic Streptomyces spp.strains according to morphological characteristics,physiological and biochemical traits,combined with 16S rDNA sequences;and analyzed further the PAI pathogenic gene composition of pathogenic strains.The results showed that there were 5 pathogenic Streptomyces species in 263 pathogenic Streptomyces strains isolated and identified from 154 potato samples collected in 6 cities,including S.scabies,S.acidicabies,S.bottropensis,S.rochei and S.lavendulae.Among them,S.scabies was the dominant strain,S.bottropensis,S.rochei and S.lavendulae were reported as potato common scab pathogen for the first time in Heilongjiang Province.Through conducting PCR amplification on pathogenicity island genes(txtAB,tomA,nec1)of pathogenic Streptomyces spp.,2 types of disease-causing gene,txtAB^(+)/tomA^(+)/nec1^(+)and txtAB^(-)/tomA^(+)/nec1^(+)were detected.

关 键 词：马铃薯疮痂病 致病链霉菌 鉴定 PAI致病基因 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]