贯彻“疾病”与“肾细胞”主线的综合性实验教学案例初探  

Preliminary Exploration of Comprehensive Experimental Teaching Cases that Implement the Main Lines of“Disease”and“Kidney Cells”

作　　者：范宇 唐昊 李小燕[1] 郑凌[1] FAN Yu;TANG Hao;LI Xiaoyan;ZHENG Ling(College of Life Sciences,Wuhan University,Wuhan,Hubei 430072,China)

机构地区：[1]武汉大学生命科学学院,湖北武汉430072

出　　处：《武汉职业技术学院学报》2022年第2期110-114,共5页Journal of Wuhan Polytechnic

基　　金：2020年中央高校改善基本办学条件专项资金武汉大学生物学教学实验室建设项目;2021年度基础学科拔尖学生培养计划2.0“生物科学拔尖人才系统科学研究训练模式研究与实践”(项目编号:20211043)。

摘　　要：急性肾损伤(acute kidney injury,AKI)指肾功能的急剧下降,表现为血清肌酐水平升高、排尿量减少,是一种常见的、严重危及患者生命的临床综合征。AKI具有较高死亡率,但缺乏有效治疗药物,是亟待解决的生物医学难题。顺铂(cisplatin)是用于治疗肺癌、宫颈癌、黑色素瘤等实体癌的最常用化疗药物,但常引起如AKI的副作用。以顺铂诱导小鼠急性肾损伤为临床常见疾病模型,设计适应于本科生的综合性动物实验教学课程,其内容包括小鼠模型制备、血清生化指标检测、肾脏组织中基因水平检测以及病理切片评估等。该课程极大培养本科生的动物实验动手能力,有助于提高学生多角度分析解决问题的能力,增加对科学研究的兴趣进而发挥学习的主观能动性。Acute kidney injury(AKI)is a common and severe life-threatening clinical syndrome which refers to a sudden decline in renal function characterized by increased serum creatinine level and decreased urine output within seven days.AKI has a high mortality rate but lacks effective drugs.Cisplatin is one of the most widely used drugs for solid cancers such as lung cancer,cervical cancer and melanoma,but it causes many side effects including AKI.Based on the cisplatin-induced AKI mouse model,a comprehensive experimental course suitable for undergraduates was designed.Hand on construction of mouse cisplatin-induced AKI model,measurements of serum biochemical indexes,evaluation of renal gene expressions and pathological changes,have been taught in this course.This course helps undergraduates to master basic animal experimental techniques,deepen their thinking about data analysis,and increase their interests in scientific research thus initiatives their learning abilities.

关 键 词：急性肾损伤 顺铂 小鼠模型 综合性实验课 

分 类 号：G642.4[文化科学—高等教育学]