In Vitro Propagation of Agave guiengola Gentry Using Semisolid Medium and Temporary Immersion Bioreactors  

作　　者：Lucía Isabel Chávez-Ortiz JoséFrancisco Morales-Domínguez Araceli Rodríguez-Sahagún Eugenio Pérez-Molphe-Balch 

机构地区：[1]Departamento de Química,Centro de Ciencias Básicas,Universidad Autónoma de Aguascalientes,Aguascalientes,20131,México [2]Laboratorio de Biología Molecular Vegetal,Centro Universitario de la Ciénega,Universidad de Guadalajara,Ocotlán,47820,México

出　　处：《Phyton-International Journal of Experimental Botany》2021年第3期1003-1013,共11页国际实验植物学杂志（英文）

基　　金：This work was supported financially by project AGS-2015-02-01-267656,Fondo Mixto CONACYT-Gobierno del Estado de Aguascalientes,and project PIBT-18-2,Universidad Autónoma de Aguascalientes,México.

摘　　要：Agave guiengola Gentry is an endemic plant from a very small locality in Oaxaca,Mexico.Its conservation status is fragile and can rapidly worsen.Because of its scarcity,this agave has been used solely for ornamental purposes,but it could have other uses if more plants were available.In vitro propagation by enhanced axillary sprouting from stem segments was attained using Murashige and Skoog Basal Medium(MS)as well as basal medium supplemented with cytokinins 6-Benzylaminopurine(BA)or 6-(γ,γ-Dimethylallylamino)purine(2iP).The best treatment for shoot induction in semisolid medium consisted in MS supplemented with 2 mg l^(–1) BA,obtaining a mean of 3.7 shoots per explant.Other interesting responses were observed,such as nodular callus induction using combinations of BA and 2,4-Dichlorophenoxyacetic acid(2,4-D);root induction without Plant Growth Regulators(PGR);and generation of shoot clusters.These clusters constituted an excellent explant for micropropagation in temporary immersion bioreactors,obtaining a propagation rate of 43 shoots per explant with 1 min immersion and 6 h immersion frequencies.All new plants rooted and survived the transfer to soil.This study developed an in vitro propagation scheme to produce individuals that can be used either for reforestation,economical purposes,or to carry out studies in this species to assess its full potential,avoiding exploitation from wild plants.

关 键 词：AGAVACEAE temporary immersion systems callus induction MICROPROPAGATION 

分 类 号：TG2[金属学及工艺—铸造]