The Potassium Transporter AtKUP12 Enhances Tolerance to Salt Stress through the Maintenance of the K^(+)/Na^(+)Ratio in Arabidopsis  

作　　者：Hua Zhang Zhongmin Yang Xilong You Youqiang Heng Yan Wang 

机构地区：[1]Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi,830046,China

出　　处：《Phyton-International Journal of Experimental Botany》2021年第2期389-402,共14页国际实验植物学杂志（英文）

基　　金：This work was supported by the National Natural Science Foundation of China[Grant No.31860061];Opening of Key Laboratory of Autonomous Region[Grant No.2017D04026];Tianshan Youth Program[Grant No.2019Q013].

摘　　要：Potassium(K^(+))is a necessary nutrient for plant growth and crop production.The K^(+)transporter plays crucial roles in the absorption and transport of K^(+)in plants.Most K^(+)transporters in Arabidopsis have been reported,but AtKUP12,which is a member of the KT/KUP/HAK family,has not yet been the subject of relevant in-depth research.In the present study,we demonstrated that AtKUP12 plays a crucial role in K^(+)uptake in Arabidopsis under 100μM low-K^(+)and 125 mM salt stress conditions.AtKUP12 transcripts were induced by K^(+)deficiency and salt stress.We analyzed the K^(+)uptake of AtKUP12 using the K^(+)uptake-deficient yeast R5421 and Arabidopsis mutant atkup12.Transformation with AtKUP12 rescued the growth defect of mutant yeast and atkup12 mutant plants at the low-K^(+)concentration,which suggested that AtKUP12 might be involved in high-affinity K^(+)uptake in low-K^(+)environments.In comparison to the wild-type(WT)and atkup12-AtKUP12 complementation lines,atkup12 showed a dramatic reduction in potassium concentration,K^(+)/Na^(+)ratio,and root and shoot growth on 12-day-old seedlings under the salt conditions;however,there was no significant difference between the complementation and WT lines.Taken together,these results demonstrate that AtKUP12 might participate in salt tolerance in Arabidopsis through K^(+)uptake and K^(+)/Na^(+)homeostasis.

关 键 词：AtKUP12 atkup12 mutant K+uptake K^(+)/Na^(+)homeostasis salt tolerance 

分 类 号：Q94[生物学—植物学]