Efficient Evergreen Plant Regeneration of Cinnamomum japonicum Sieb.through in vitro Organogenesis  

作　　者：Jiaji Zhang Xiaofei Long Yuhao Weng Tielong Cheng Jisen Shi Jinhui Chen 

机构地区：[1]Key Laboratory of Forest Genetics&Biotechnology of Ministry of Education,Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing,210037,China [2]College of Biology and the Environment,Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing,210037,China

出　　处：《Phyton-International Journal of Experimental Botany》2021年第2期571-582,共12页国际实验植物学杂志（英文）

基　　金：This research is supported by Key research and development plan of Jiangsu Province(BE2017376);Foundation of Jiangsu forestry bureau(LYKJ[2017]42);the Qinglan project of Jiangsu province and Priority Academic Program Development of Jiangsu Higher Education Institutions to J.H.Chen,and the Nature Science Foundation of China(31770715)to T.L.Cheng.

摘　　要：Cinnamomum japonicum Sieb.is an excellent roadside tree and medicinal tree species with considerable ornamental and economic value.In this study,we successfully developed a large-scale micropropagation protocol for C.japonicum for the first time.Sterilized shoots were excised and used as explants for shoot induction on several basal media,supplemented with different concentrations of plant growth regulators(PGRs),such as Thidiazuron(TDZ),N^(6)-Benzyladenine(6-benzylaminopurine)(BA),α-naphthaleneacetic acid(NAA)and Gibberellic acid(GA_(3)).After comparison,the most efficient medium for shoot regeneration was 1/2 Murashige and Skoog(MS)medium containing 0.5 mg L^(-1)BA,0.05 mg L^(-1)NAA and 0.2 mg L^(-1)GA_(3),which resulted in an average number of induced shoots per explant and shoot length of 5.2 and 1.62 cm at 28 d,respectively.Then,elongated adventitious shoots were transferred to induce roots.86.7%of shoots was able to root on 1/2 MS medium supplemented with 0.5 mg L^(-1)NAA and 0.1 mg L^(-1)BA.The earliest rooting time observed was after 21 d and the average root length was up to 3.3 cm after 28 d.Our study shows that C.japonicum can be successfully regenerated through de novo organogenesis,which lays a foundation for future transformation research on this tree.

关 键 词：Cinnamomum japonicum Sieb. MICROPROPAGATION de novo organogenesis shoot induction RHIZOGENESIS 

分 类 号：Q94[生物学—植物学]