Benefit of prophylactic bronchodilator withβ2 adrenergic agonist in ischemia-reperfusion-induced lung injury  

作　　者：CHEN-LIANG TSAI YU-HUEI LIN CHIH-YING CHANGCHIEN CHIH-FENG CHIAN CHI-HUEI CHIANG 

机构地区：[1]Division of Pulmonary and Critical Care,Department of Internal Medicine,Tri-Service General Hospital,National Defense Medical Center,Taipei,114,Taiwan [2]Post-Baccalaureate Program in Nursing,College of Nursing,Taipei Medical University,Taipei,110,Taiwan [3]Department of General Medicine,Tri-Service General Hospital,National Defense Medical Center,Taipei,114,Taiwan [4]Division of Pulmonary Immunology and Infectious Diseases,Chest Department,Taipei Veterans General Hospital,Taipei,112,Taiwan

出　　处：《BIOCELL》2021年第5期1201-1211,共11页生物细胞（英文）

基　　金：the Tri-Service General Hospital,National Defesnse Medical Center in Taiwan(TSGH-C108-109).

摘　　要：Primary lung graft dysfunction could significantly attribute to ischemia-reperfusion lung injury(IRLI)during transplantation surgery.β2-adrenergic agonists were one of the bronchodilators that had been well-established in the management of asthma and chronic obstructive pulmonary disease(COPD)with anti-inflammatory potency.By applying the model of isolated rat lung,we evaluated the efficacy of short-actingβ2-agonist inhalation to ameliorate ischemia-reperfusion damage.The experiment protocol was 180 min of global ischemia and then reperfusion for 60 min.In theβ2-agonist inhalation group,aerosolized albuterol was administrated prior ischemia procedure.Increased weight ratios of wet to dry lung and microvascular permeability were characterized in the IRLI group.In contrast,pre-inhaledβ2-agonist significantly mitigated the severity of pulmonary edema.Bronchoalveolar lavage from theβ2-agonist group presented decreased leukocyte counts and cytokines production,including interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),and macrophage inflammatory protein 2(MIP-2).Devastating oxidative stress was widely recognized during the ischemia-reperfusion process,whileβ2-agonist pretreatment revealed subsided H2O2,myeloperoxidase(MPO),and the cleavage of caspase-3.Western blotting from lung homogenates identified the blockade of NF-κB and MAPK activation in theβ2-agonist inhalation group.Currently,there was no specific pharmacotherapy in IRLI management.Our results elucidated the protective effect ofβ2-agonist bronchodilator against ischemia-reperfusion induced oxidative stress,inflammation reaction,and pulmonary edema.

关 键 词：Ischemia-reperfusion lung injury β2-adrenergic agonist BRONCHODILATOR Lung transplantation 

分 类 号：R56[医药卫生—呼吸系统]