OGP对低氧环境中小鼠BMSCs成脂分化影响的研究  

Effects of osteogenic growth peptide combined with hypoxia on the adipogenice differentiation of mouse bone marrow mesenchymal stem cells

作　　者：张磊[1] 龚跃昆[2] 尹勇 ZHANG Lei;GONG Yuekun;YIN Yong(Department of Rehabilitation Medicine, the Affiliated Hospital of Yunnan University, Kunming 650000, China;Department of Orthopaedics,the First Affiliated Hospital of Kunming Medical University, Kunming 650000, China)

机构地区：[1]云南大学附属医院康复医学科,云南昆明650000 [2]昆明医科大学第一附属医院骨科,云南昆明650000

出　　处：《中国骨质疏松杂志》2022年第5期670-674,共5页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金(81760392);云南省医学学科后备人才项目(H-2017057);云南省科技厅-昆明医科大学联合专项(2018FE001-108)。

摘　　要：目的检测成骨生长肽(osteogenic growth peptide,OGP)与低氧环境对小鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)成脂肪分化的影响。方法实验随机分为4组:A组(常氧组)、B组(1%O_(2)组)、C组(常氧+10^(-9)mol/L OGP组)、D组(1%O_(2)+10^(-9)mol/L OGP组)。ELISA法定量检测低氧诱导因子1α(hypoxia inducible factor 1α,HIF-1α)蛋白的表达;14 d后,油红O染色观察BMSCs脂滴形成情况;实时荧光定量PCR和Western blotting检测成脂相关基因PPAR-γ和C/EBPα的mRNA和蛋白表达水平。结果与A组及C组相比,B组与D组HIF-1α表达明显上调(P<0.01);与A组相比,B组、C组、D组脂滴生成及PPAR-γ和C/EBPα的mRNA和蛋白表达降低(P<0.01);与B组及C组相比,D组中脂滴生成及PPAR-γ和C/EBPα的信使RNA和蛋白表达下调(P<0.01)。结论OGP及1%低氧环境均能抑制BMSCs成脂分化,且二者抑制BMSCs成脂分化具有协同效应。Objective To investigate the effects of osteogenic growth peptide conbined with physical hypoxia on the adipogenic differentiation of mouse bone marrow mesenchymal stem cells.Methods The study was randomly divided into four groups:group A(under normal oxygen level);group B(under 1%oxygen level);group C(with 10^(-9)mol/L OGP under normal oxygen level);group D(with OGP under 1%oxygen level).Cells were collected at 24 h to detect the hypoxia inducible factor 1α(HIF-1α)through ELISA.At 14 d,cells were collected to observe the formation of lipid droplets in the cells and to detect the mRNA and protein level of PPAR-γand C/EBPαusing qPCR and western-blotting.Results Compared with group A and group C,the expression of HIF-1αin the group B and group D upregulated obviously.Compared with that in group A,the lipid droplets accumulation increased and the expression of PPAR-γand C/EBPαon the mRNA and protein level upregulated in the group B,group C and group D;compared with that in group B and group,the lipid droplets accumulation increased and the expression of PPAR-γand C/EBPαon the mRNA and protein level upregulated in the group D.Conclusion OGP and hypoxia can both inhibit the BMSCs adipogenesis and OGP plus hypoxia have co-effect to inhibit the adipogenesis of BMSCs.

关 键 词：骨髓间充质干细胞 低氧 成骨生长肽 成脂分化 

分 类 号：R683[医药卫生—骨科学] R681[医药卫生—外科学]