健康与患根腐病草莓根际、非根际及根内细菌群落多样性  

Bacterial community diversity in rhizosphere,non-rhizosphere and root tissue of healthy and root rot strawberry plants

作　　者：戴瑞卿 赖宝春 曾天宝 吴振强 王家瑞 DAI Rui-qing;LAI Bao-chun;ZENG Tian-bao;WU Zhen-qiang;WANG Jia-rui(Zhangzhou Institute of Agricultural Sciences of Fujian Province,Zhangzhou,Fujian 363005,China)

机构地区：[1]福建省漳州市农业科学研究所,福建漳州363005

出　　处：《西南农业学报》2022年第4期906-914,共9页Southwest China Journal of Agricultural Sciences

基　　金：福建省科技计划项目(2019N0053)。

摘　　要：【目的】明确健康与患根腐病草莓根际土壤、非根际土壤以及根内细菌群落组成与多样性。【方法】采用Illumina Miseq高通量测序技术,分别对草莓根际土壤、非根际土壤及根内细菌16S rRNA基因的V3~V4区进行扩增并测序,分析健康与患根腐病草莓根际土壤、非根际土壤及根内细菌群落结构与多样性。【结果】健康与患根腐病草莓土壤及根内细菌多样性丰富,健康草莓组共鉴定出细菌30个门、78个纲、131个目、238个科、489个属,患根腐病草莓组共鉴定出细菌30个门、78个纲、131个目、233个科、483个属。门水平上,变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)和酸杆菌门(Acidobacteria)为根际和非根际土壤的优势菌门,放线菌门和拟杆菌门的相对丰度在健康与患根腐病草莓根际、非根际土壤中存在差异。根内样品中的优势菌门为变形菌门和蓝细菌门(Cyanobacteria),二者的相对丰度在健康与患根腐病草莓根内存在显著性差异。属水平上,鞘氨醇单胞菌属(Sphingomonas)、新鞘氨醇杆菌属(Novosphingobium)、伯克霍尔德菌属(Burkholderia)、链霉菌属(Streptomyces)、芽单胞菌属(Gemmatimonas)和芽孢杆菌属(Bacillus)为根际和非根际土壤的优势菌属,且在健康与患根腐病草莓土壤中各优势菌属的相对丰度均存在显著性差异。根内样品的优势属为链形植物属(Streptophyta),其相对丰度在健康与患根腐病草莓根内存在显著性差异。样本比较分析表明,健康与患根腐病草莓根内细菌群落结构差异大于根际和非根际土壤。【结论】草莓植株发生根腐病后根际土壤和根内细菌群落丰富度和多样性均有提高,而非根际土壤细菌群落丰富度和多样性下降。健康与患根腐病草莓根际、非根际土壤及根内细菌群落组成差异性显著,其中链形植物属、链霉菌属、伯克霍尔德菌属和芽孢杆菌属等可�【Objecctive】The present paper aimed to confirm the bacterial community composition and diversity of healthy and root rot strawberry in rhizosphere soil, non-rhizosphere soil and root tissue.【Method】The V3-V4 region of the bacterial 16 S rRNA gene in rhizosphere soil, non-rhizosphere soil and root tissue was amplified, the amplified fragments were sequenced by Illumina MiSeq high-throughput sequencing technology, and their bacterial community composition and diversity were analyzed.【Result】The microbial diversity was rich in the soils and root tissue of strawberry. Healthy and root rot strawberry group samples got 30 phyla, 78 classes, 131 orders, 238 families, 489 genera and 30 phyla, 78 classes, 131 orders, 233 families, 483 genera, respectively. At the phylum level, Proteobacteria, Bacteroidetes, Actinobacteria and Acidobacteria were the dominant phyla in all rhizosphere and non-rhizosphere soil. The relative abundance of Actinobacteria and Bacteroidetes in rhizosphere and non-rhizosphere soil was different from that of healthy and root rot strawberry. Proteobacteria and Cyanobacteria were the dominant phyla in all root tissue. The relative abundance of Proteobacteria and Cyanobacteria in root tissue was significantly different from that of healthy and root rot strawberry. At the genus level, the dominant genera in all rhizosphere and non-rhizosphere soil were Sphingomonas, Novosphingobium, Burkholderia, Streptomyces, Gemmatimonas and Bacillus. The relative abundance of dominant genera was significantly different between healthy and diseased groups. The dominant genus in all root tissue was Streptophyta, which relative abundance in root tissue was significantly different from that of healthy and diseased groups. The analysis of samples comparison showed that the difference of bacterial community structure in root tissue was greater than that of rhizosphere and non-rhizosphere soil.【Conclusion】Bacterial richness and diversity in the rhizosphere soil and root tissue increased, of which in the non-

关 键 词：草莓根腐病 细菌多样性 群落结构 高通量测序 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]