玉米穗位叶快速叶绿素荧光参数及产量相关性状QTL定位  

QTL Mapping for Fast Chlorophyll Fluorescence Parameters of Ear Leaves and Yield-related Traits in Maize

作　　者：刘欢欢 王艳[1] 陈婉莹 汪欢 毕兴林 何永辉 印志同[1,2] Liu Huanhuan;Wang Yan;Chen Wanying;Wang Huan;Bi Xinglin;He Yonghui;Yin Zhitong(Jiangsu Key Laboratory of Crop Genetics and Physiology,Agricultural College of Yangzhou University,Key Laboratory of Plant Functional Genomics of the Ministry of Education,Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding,Yangzhou,225009;Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou University,Yangzhou,225009)

机构地区：[1]扬州大学农学院,江苏省作物基因组学和分子育种重点实验室,植物功能基因组学教育部重点实验室,江苏省作物遗传生理重点实验室,扬州225009 [2]扬州大学,江苏省粮食作物现代产业技术协同创新中心,扬州225009

出　　处：《分子植物育种》2022年第6期1872-1882,共11页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31901551);中国博士后科学基金项目(2020M681745);江苏省作物学优势学科项目共同资助。

摘　　要：穗位叶的光合速率是影响玉米产量形成的重要因素,而快速叶绿素荧光参数可动态高效的反映光合生理变化及能量传递情况,因此解析调控穗位叶叶绿素荧光参数的遗传基础对于开展玉米高光效育种具有重要意义。本研究利用LDC-1×YS501衍生的重组自交系(recombinant inbred line,RIL)群体,结合高密度分子遗传图谱在两个环境条件下对7个叶绿素荧光参数及5个产量相关性状进行QTL(Quantitative trait locus)定位。结果共检测到51个调控荧光参数的QTLs,这些QTLs在1、2、3、6、10号染色体的部分区段显著富集,且其中5个位点qET2-1、qET10-1、qPI10-1、qTC3-1和qEC2-1在两个环境下均能检测到;共检测到13个产量相关性状的QTLs,其中位于1号染色体上qER1-1与调控荧光参数PICS的位点qPI1-2区间重叠。本研究将为后续基因挖掘和开展分子标记辅助育种提供重要基础。The photosynthetic rate of ear leaves is an important factor affecting maize yield,and fast chlorophyll fluorescence parameters can reflect the physiological changes and energy transfer of photosynthesis dynamically and efficiently.Therefore,it is of great significance to analyze the genetic basis of regulating chlorophyll fluorescence parameters of ear leaves for maize high-photosynthetic-efficiency breeding.In this research,using a recombinant inbred lines population derived from YS501×LDC-1,QTL(quantitative trait locus)mapping of 7 chlorophyll fluorescence parameters and 5 yield-related traits was carried out under two environmental conditions.A total of 51 QTLs regulating fluorescence parameters were detected.These QTLs were significantly enriched in some segments of chromosomes 1,2,3,6,and 10 and 5 loci,qET2-1,qET10-1,qPI10-1,qTC3-1 and qEC2-1 could be detected in both environments.13 QTLs for yield-related traits were detected,among which qER1-1 on chromosome 1 overlapped with qPI1-2,a locus regulating fluorescence parameter PICS.This research will provide an important foundation for subsequent gene cloning and molecular marker-assisted breeding.

关 键 词：玉米 穗位叶 快速叶绿素荧光参数 QTL定位 

分 类 号：S513[农业科学—作物学]