AstragalosideⅣimproves melanocyte differentiation from mouse bone marrow mesenchymal stem cells  

作　　者：XINGYU MEI ZHOUWEI WU CHENGZHONG ZHANG YUE SUN WEIMIN SHI 

机构地区：[1]Department of Dermatology,Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200080,China.

出　　处：《BIOCELL》2021年第6期1551-1559,共9页生物细胞（英文）

基　　金：the National Natural Science Foundation of China(Grant No.81703140).

摘　　要：Vitiligo results in an autoimmune disorder destructing skin pigment cells,melanocytes(Mcs).This study aimed to investigate whether Astragaloside IV(AIV)could efficiently induce differentiation of bone marrow mesenchymal stem cells(BMMSCs)into Mcs.BMMSCs were induced and differentiated into Mcs with 0.1,0.2,and 0.4 mg/L AIV during 150-day.Morphologic changes of differentiated cells were observed.Levels of some melanocytic specific genes(TRP-1,TRP-2,MART-1,Mitf)were measured with quantitative polymerase chain reaction(qPCR)at 90,120,and 150 days of induction.After 90-day induction,the differentiated cells with 0.4 mg/L AIV demonstrated the typical morphology of Mcs,positive 3,4 dihydroxyphenylalanine staining,and positive staining of TRP-1,TRP-2,MART-1,and Mitf.After 90-and 120-days’induction with 0.4 mg/L AIV,TRP-1 expression was significantly elevated(p<0.01),and TRP-2 expression was significantly increased in 0.4 mg/L AIV-treated group compared to negative control(p<0.01),0.1 mg/L(p<0.01),and 0.2 mg/L(p<0.01)AIV-treated groups.Moreover,MART-1 expression was significantly up-regulated in 0.4 mg/L AIV-treated group compared to negative control,but without difference compared to 0.1 mg/L(p>0.05)and 0.2 mg/L(p>0.05)AIV-treated groups.During 90 to 150-day induction,there were no significant differences for Mitf levels between AIV-treated groups and negative control(p>0.05).In conclusion,90-day induction with 0.4 mg/L AIV up-regulated TRP-1,TRP-2,and MART-1 expression,indicating that AIV can efficiently induce Mcs differentiation from BMMSCs.These results provide experimental and theoretic evidence for AIV application in clinical vitiligo repigmentation treatment.

关 键 词：Tyrosinase-related protein Melanoma antigen recognized by T cells 1 Microphthalmia-associated transcription factor Committed differentiation Traditional Chinese medicine 

分 类 号：S85[农业科学—兽医学]