Doubled haploid production in advanced back cross generations and molecular cytogenetic characterization of rye chromatin in triticale×wheat derived doubled haploid lines  

作　　者：MUNIYANDI SAMUEL JEBERSON HARINDER KUMAR CHAUDHARY RAKESH KUMAR CHAHOTA SHABIR HUSSAIN WANI 

机构地区：[1]AICRP(MULLaRP),Directorate of Research,CAU,Imphal,India [2]Molecular Cytogenetics and Tissue Culture Laboratory,CSKHPKV,Palampur,India [3]Department of Agricultural Biotechnology,CSKHPKV,Palampur,India [4]Mountain Research Center for Field Crops,Sher-e-Kashmir University of Agricultural Sciences and Technology,Khudwani,India

出　　处：《BIOCELL》2021年第6期1651-1659,共9页生物细胞（英文）

摘　　要：The rye genome has shown potential for improvement of bread wheat,where wheat-rye substitutions and translocations have been and are frequently used in resistance breeding.Crosses belongs to different generations viz.,BC_(1)F_(1),BC_(1)F_(2),BC_(1)F_(3),BC_(1)F_(4)and BC_(2)F_(3)of triticale×wheat derived were used for different haploid induction parameters using Gogon grass(Imperata cylindrica)as a pollen source.The percentage of pseudo seed formation ranged from 34.55%for BC_(1)F_(2)to 63.77 for BC_(1)F_(1)crosses,the haploid embryo formation ranges from 9.43%for BC_(1)F_(1)to 30.2%for BC_(1)F_(2),the haploid plant generation ranges from 19.36%for BC_(1)F_(2)to 63.25%for BC_(1)F_(1).Four doubled haploids were developed from ITSN 105/58-VL 802-VL 802 of BC_(2)F_(3)underwent molecular cytogenetic analyses using the probes,viz.,rye genomic rDNA,pSc 119 and pAs1.FISH and GISH analysis revealed an IBL.1RS translocation and substitution of 5R chromosome instead of the 5D chromosomes in these doubled haploids.

关 键 词：HAPLOID Triticale×wheat backcross FISH GISH Rye chromatin 

分 类 号：S51[农业科学—作物学]