糖复康方对KK-Ay小鼠肠道菌群的影响及其治疗效果观察  

Effect of Tangfukang Formula on intestinal microflora of KK-Ay mice and its therapeutic effect

作　　者：王燕[1] 高慧娟 闫凯[3] 王威 冯兴中 WANG Yan;GAO Hui-juan;YAN Kai;WANG Wei;FENG Xing-zhong(Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Tsinghua University Yuquan Hospital,Endocrinology Department,Beijing 100040;TCM Department,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100043;Beijing Shijitan Hospital affiliated to Capital Medical University,Beijing 100038;Tsinghua University Yuquan Hospital,Beijing 100040)

机构地区：[1]天津中医药大学,天津301617 [2]清华大学玉泉医院内分泌免疫科,北京100040 [3]首都医科大学附属北京朝阳医院中医科,北京100043 [4]首都医科大学附属北京世纪坛医院,北京100038 [5]清华大学玉泉医院,北京100040

出　　处：《北京中医药》2022年第3期230-235,共6页Beijing Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(82104812)。

摘　　要：目的探讨糖复康方对KK-Ay小鼠降糖的疗效及其与肠道菌群之间复杂的生物网络关系。方法将8周龄SPF级24只KK-Ay自发糖尿病模型小鼠随机分为模型组、糖复康方高剂量组、糖复康方等剂量组、二甲双胍组4组,各6只;6只C57BL/6J小鼠作为正常对照组。正常对照组及模型组每日给予生理盐水灌胃,糖复康方高剂量组、等剂量组分别给予糖复康方6.4、3.2 g/(kg·d)灌胃,二甲双胍组给予二甲双胍250 mg/(kg·d)灌胃。均干预1个月后取材,同时检测小鼠血糖,光学显微镜下观察骨骼肌细胞病变程度,采用16S r DNA测序法分析小鼠肠道菌群的结构。结果糖复康方可有效降低小鼠血糖,减轻骨骼肌炎症浸润及萎缩坏死,增加小鼠的肠道菌群的多样性,拟杆菌相对丰度增加,而厚壁菌门的相对丰度降低。结论糖复康方对KK-Ay小鼠具有良好的降糖作用,其治疗作用可能与调节肠道菌群结构相关。Objective To investigate the hypoglycemic effect of Tangfukang Formula on KK-Ay mice and its complex biological network relationship with intestinal flora.Methods Twenty-four 8-week-old SPF KK-Ay spontaneous diabetes model mice were randomly divided into 4 groups,namely model group,Tangfukang high-dose group,equivalent dose group and metformin group,with 6 mice in each group.Six C57BL/6J mice were used as normal control group.Normal control group and model group were given normal saline with intragastric administration,Tangfukang high-dose group,equivalent dose group and metformin group were given Tangfukang of 6.4 and 3.2 g/kg·d and metformin of 250 mg/kg·d with intragastric administration respectively.After 1 month of intervention,the sample was got and the blood glucose was detected,the pathological degree of skeletal muscle cells was observed under light microscope,and the structure of intestinal microflora of mice was analyzed by 16S rDNA sequencing.Results Tangfukang Formula could effectively reduce blood glucose,alleviate inflammatory infiltration and atrophy and necrosis of skeletal muscle,and significantly increase the diversity of intestinal flora,increase the relative abundance of bacteroidetes and decrease the relative abundance of firmicutes in mice.Conclusion Tangfukang Formula has a good hypoglycemic effect on KK-Ay mice,and its antidiabetic effect may be related to the regulation of intestinal microflora structure.

关 键 词：糖复康方 KK-AY小鼠 肠道菌群 

分 类 号：R285.5[医药卫生—中药学]