糖络宁通过调控lncRNA MALAT1减轻高糖诱导的雪旺细胞炎症反应机制研究  

Mechanism of Tangluoning for alleviating high glucose-induced inflammatory reaction of Schwann cells by regulating lncRNA MALAT1

作　　者：王晓磊 高彦彬[2] 孙宏峰[1] 吴淑馨[1] 胡燕[1] 王颖[1] 胡浩 张涛静[1] WANG Xiao-lei;GAO Yan-bin;SUN Hong-feng;WU Shu-xin;HU Yan;WANG Ying;HU Hao;ZHANG Tao-jing(Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China;Beijing Key Laboratory on TCM Collateral Diseases,College of Traditional Chinese Medicine,Capital Medical University,Beijing 100069)

机构地区：[1]北京中医药大学东方医院,北京100078 [2]首都医科大学中医药学院中医络病研究北京市重点实验室,北京100069

出　　处：《北京中医药》2022年第3期236-239,共4页Beijing Journal of Traditional Chinese Medicine

基　　金：国家中医药管理局第六批全国老中医药专家学术经验继承工作项目(国中医药人教发[2017]29号);中央高校基本科研业务费专项资金(2020-JYB-XJSJJ-057);北京中医药大学校级杰出青年人才项目(BUCM-2019-JCRC010);2021年度中医络病研究北京市重点实验室开放课题资助。

摘　　要：目的以lncRNA MALAT1为切入点,观察糖络宁对体外高糖诱导的大鼠雪旺细胞炎症反应的影响,探讨其保护雪旺细胞的可能机制。方法以大鼠雪旺细胞作为研究对象,采用高糖刺激构建细胞损伤模型,分为正常组(空白血清培养)、高糖组(150 mmol/L葡萄糖+空白血清培养)、糖络宁组(150 mmol/L葡萄糖+糖络宁含药血清培养)、MALAT1基因敲除组(150 mmol/L葡萄糖+MALAT1抑制剂)4个组,48 h后,收集各组细胞并进行相关检测。以CCK-8法检测各组细胞活力,酶联免疫法检测各组细胞上清液中炎症因子(IL-6、MCP-1、TNF-α)的蛋白表达情况,实时荧光定量聚合酶链式反应检测各组细胞lncRNA MALAT1和炎症因子(IL-6、MCP-1、TNF-α)的表达情况。结果与正常组比较,高糖组细胞活力下降(P<0.05),炎症因子(IL-6、MCP-1、TNF-α)的基因和蛋白表达均增加(P<0.05),MALAT1表达水平明显升高(P<0.05);而与高糖组相比,糖络宁组和MALAT1基因敲除组细胞活力有所升高(P<0.05),各炎症因子的基因和蛋白表达均有所下调(P<0.05),且糖络宁组MALAT1的表达水平较高糖组明显减少(P<0.05)。结论糖络宁能减轻高糖诱导的大鼠雪旺细胞炎症反应,其部分机制是通过抑制lncRNA MALAT1的水平实现的。Objective To observe effects of Tangluoning on inflammatory reaction of Schwann cells induced by high glucose in vitro with lncRNA MALAT1 as the starting point and explore the possible mechanism of protecting Schwann cells.Methods With Schwann cells of rats as objective,cell injury model was established by high glucose stimulation.They were randomly divided into 4 group:normal group(blank serum culture),high glucose group(blank serum culture+150 mmol/L glucose),Tangluoning group(medicine serum culture+150 mmol/L glucose)and lncRNA MALAT1 knockout group(150 mmol/L glucose+MALAT1 inhibitor).The cells of each group were collected and detected after 48 hours.Cells viability was determined by CCK-8 assay.Expression of lncRNA MALAT1 and inflammatory factors(IL-6,MCP-1 and TNF-α)were assessed by real-time PCR or enzyme linked immunosorbent assay.Results Compared with the normal group,the cell viability of high glucose group was reduced(P<0.05),the gene and protein expression of inflammatory factors(IL-6,MCP-1 and TNF-α)were increased(P<0.05),the expression of MALAT1 was increased(P<0.05);compared with high glucose group,the cell viability of both Tangluoning group and MALAT1 knockout group was increased(P<0.05),the gene and protein expression of inflammatory factors were decreased(P<0.05),the expression of MALAT1 of Tangluoning group was more decreased than that of the high glucose group(P<0.05).Conclusion Tangluoning could alleviate inflammatory reaction of Schwann cell induced by high glucose through inhibiting the expression of lncRNA MALAT1.

关 键 词：糖络宁 雪旺细胞 lncRNA MALAT1 炎症反应 大鼠 

分 类 号：R285.5[医药卫生—中药学]