舒脉胶囊抑制大鼠髂动脉介入术后内膜增生的作用机制  

Mechanism of Shumai Capsule in inhibiting intimal hyperplasia after iliac artery intervention in rats

作　　者：谭崇赋 徐旭英[1] TAN Chong-fu;XU Xu-ying(Vascular Surgery of Sore and Ulcer,Beijing Hospital of Traditional Chinese Medicine Affiliated to the Capital Medical University,Beijing 100010,China)

机构地区：[1]首都医科大学附属北京中医医院疮疡血管外科,北京100010

出　　处：《北京中医药》2022年第3期255-259,共5页Beijing Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81873118)。

摘　　要：目的探讨舒脉胶囊抑制大鼠髂动脉介入术后内膜增生的作用机制。方法28只大鼠随机分为假手术组、模型组、舒脉胶囊组、FABP4抑制剂组,每组7只。假手术组只做皮肤和肌层切开,不做球囊牵拉,模型组、舒脉胶囊组、FABP4抑制剂组均用经皮冠状动脉腔内血管成形术(PTCA)扩张导管,经左股动脉损伤左髂动脉建立下肢动脉再狭窄模型。术后第2天,舒脉胶囊组给予0.068 g/mL舒脉胶囊悬浊液灌胃,FABP4抑制剂组以浓度为1.6 mg/kg腹腔注射,假手术组和模型组给予生理盐水灌胃,1次/d。6周后,以小动物多普勒超声检测各组左髂动脉内膜厚度、管腔直径;ELISA法检测血清一氧化氮(NO)、FABP4水平变化;采用Western blotting法检测左髂动脉血管组织中FABP4蛋白表达。结果模型组、舒脉胶囊组、FABP4抑制剂组左髂动脉内膜增厚,内膜厚度和管腔直径均大于假手术组(P<0.05),并且舒脉胶囊组内膜厚度小于模型组(P<0.05);模型组、舒脉胶囊组、FABP4抑制剂组血清NO水平低于假手术组(P<0.05),舒脉胶囊组、FABP4抑制剂组血清NO水平高于模型组(P<0.05);舒脉胶囊组、FABP4抑制剂组血清FABP4水平低于模型组(P<0.05);舒脉胶囊组、FABP4抑制剂组中左髂动脉血管组织中FABP4蛋白低于模型组(P<0.05)。结论舒脉胶囊可能通过靶向下调FABP4、提高NO的生成,抑制介入术后血管内膜增生,从而改善血管内皮功能。Objective To investigate the mechanism of Shumai Capsule in inhibiting intimal hyperplasia after iliac artery intervention in rats.Methods 28 rats were randomly divided into sham operation group,model group,Shumai Capsule group and FABP4 inhibitor group,with 7 rats in each group.In the sham-operated group,only skin and muscularis incision was performed,but balloon traction was not performed.The model group,Shumai Capsule group and FABP4 inhibitor group were treated with percutaneous transluminal coronary angioplasty(PTCA)catheter,and the left iliac artery was injured through the left femoral artery to establish the lower extremity artery restenosis model.On the second day after operation,Shumai Capsule group was given 0.068 g/mL Shumai Capsule suspension by gavage,and the FABP4 inhibitor(BMS309403)group was given intraperitoneal injection of 1.6 mg/kg,the sham operation group and model group were given normal saline by gavage.After 6 weeks,the intimal thickness and lumen diameter of left iliac artery were detected by small animal Doppler ultrasound,and the levels of NO and FABP4 in serum were detected by ELISA.The expression of FABP4 protein in left iliac artery was detected by Western blotting.Results The intima of model group,Shumai Capsule group and FABP4 inhibitor group was thickened and the thickness and lumen diameter of them were significantly larger than that of sham group(P<0.05),the thickness of Shumai Capsule group was less than that of model group(P<0.05).The content of NO in model group,Shumai capsule group and FABP4 inhibitor group was lower than that in sham group(P<0.05);the content of NO in Shumai capsule group and FABP4 inhibitor group was higher than that in model group(P<0.05);the content of FABP4 in Shumai Capsule group and FABP4 inhibitor group was lower than that in model group(P<0.05).The FABP4 protein in left iliac artery in Shumai capsule group and FABP4 inhibitor group was lower than that in model group(P<0.05).Conclusion Shumai Capsule can improve vascular endothelial function by down

关 键 词：外周动脉疾病 介入术后再狭窄 内膜增生 舒脉胶囊 大鼠 

分 类 号：R285.5[医药卫生—中药学]