高效液相色谱-荧光检测法测定西藏饮用天然水中阴离子表面活性剂  

Determination of Anionic Surfactant in Natural Drinking Water in Tibet by High Performance Liquid Chromatography with Fluorescence Detection

作　　者：王君 王顺芝 魏霜 曹晓钢 李国成 李志勇 WANG Jun;WANG Shun-zhi;WEI Shuang;CAO Xiao-gang;LI Guo-cheng;LI Zhi-yong(Lhasa Customs Technology Center,Lhasa 850002,China;State Key Laboratory of Mineral Water Testing(Lhasa),Lhasa 850002,China;Guangzhou Customs Technology Center,Guangzhou 510623,China;Lhasa Riduo Hot Spring Villa Co.,Ltd.,Lhasa 850216,China)

机构地区：[1]拉萨海关技术中心,西藏拉萨850002 [2]国家矿泉水检测重点实验室(拉萨),西藏拉萨850002 [3]广州海关技术中心,广东广州510623 [4]拉萨日多温泉山庄有限公司,西藏拉萨850216

出　　处：《食品与药品》2022年第3期218-221,共4页Food and Drug

摘　　要：目的建立高效液相色谱-荧光法测定西藏饮用天然水中阴离子表面活性剂含量的分析方法。方法采用Shim-pack VP-ODS C_(18)色谱柱(150 mm×4.6 mm,5μm),流动相为甲醇:水=94:6(v:v),流速0.4 ml/min,柱温40℃,检测激发波长232 nm,发射波长290 nm,外标法定量检测西藏饮用天然水中阴离子表面活性剂含量。结果阴离子表面活性剂浓度在20.0~300.0μg/L与色谱峰面积具有良好的线性关系,线性相关系数r=0.9999。方法的检出限为5.0μg/L,样品的加标回收率为96.5%~99.9%,相对标准偏差为0.2%~1.7%。结论该方法简便、快速、准确,精密度好,检出限低,可用于测定西藏饮用天然水中阴离子表面活性剂含量,值得推广应用。Objective To establish a method for the determination of anionic surfactant in natural drinking water in Tibet by high performance liquid chromatography with fluorescence detection.Methods A Shim-pack VP-ODS C_(18) chromatographic column(150 mm×4.6 mm,5μm)was adopted,the mobile phase was methanol:water of 94:6(v:v)with the flow rate of 0.4 ml/min,column temperature of 40℃,detection wavelength of 232 nm and emission wavelength of 290 nm.The content of anionic surfactant was detected by external standard method.Results There was a good linear relationship between the peak area and the concentration of anionic surfactant in the range of 20.0-300.0μg/L,and the linear correlation coefficient was 0.9999.The detection limit of the method was 5.0μg/L.The recoveries were 96.5%-99.9%,and the relative standard deviations were 0.2%-1.7%.Conclusion The method is simple,rapid and accurate,with good precision and low detection limit.It can be used to determine the content of anionic surfactant in natural drinking water in Tibet and it is worthy of popularization and application.

关 键 词：高效液相色谱-荧光法 西藏饮用天然水 阴离子表面活性剂 

分 类 号：O656.3[理学—分析化学]