Mechanisms of antimicrobial peptides as characterized by solid-state NMR  

作　　者：Rong Han Shenlin Wang 

机构地区：[1]College of Chemistry and Molecular Engineering and Beijing NMR Center,Peking University,Beijing,100871,China [2]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai,200237,China [3]Beijing National Laboratory for Molecular Science,Beijing,100871,China

出　　处：《Magnetic Resonance Letters》2022年第2期119-129,I0003,共12页磁共振快报（英文）

基　　金：supported by the National Key Research and Development Program of the Ministry of Science and Technology,People's Republic of China(contract number 2016YFA0501203);the National Natural Science Foundation of China(21874004,31470727);the Interdisciplinary Medicine Seed Fund of Peking University,and the Fundamental Research Funds for the Central University.

摘　　要：Antimicrobial peptides(AMP)are small proteins that play critical roles in host defense against microbe invasion.Many AMPs disrupt the cellular membrane of microbe,while the mechanism of action of AMPs can be very sophisticated.Solid-state NMR(SSNMR)technique is powerful in characterizing the mechanism of AMPs in vivo and in vitro.This review summarizes the recent advance of SSNMR technique in AMP mechanisms characterization.We highlight the sample preparation approaches,the SSNMR spectroscopic methods,and a number of outstanding examples of AMP mechanisms elucidated via SSNMR spectroscopy.

关 键 词：Antimicrobial peptides(AMP) Solid-state NMR(SSNMR) Sample preparation Antimicrobial mechanisms Whole-cell NMR 

分 类 号：TQ3[化学工程]