丛枝菌根(AM)真菌扩培技术研究进展  

Propagation of Arbuscular Mycorrhizal Fungi:A Review

作　　者：李少杰 肖清山 宋福强[1,2] 王歆[1,2,3] LI Shaojie;XIAO Qingshan;SONG Fuqiang;WANG Xin(Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,School of Life Sciences,Heilongjiang University,Harbin 150500;Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region,School of Life Sciences,Heilongjiang University,Harbin 150080;Key Laboratory of Molecular Biology,College of Heilongjiang,Heilongjiang University,Harbin 150080)

机构地区：[1]黑龙江大学生命科学学院,农业微生物技术教育部工程研究中心,哈尔滨150500 [2]黑龙江大学生命科学学院,黑龙江省寒地生态修复与资源利用重点实验室,哈尔滨150080 [3]黑龙江大学生命科学学院,黑龙江省普通高校分子生物学重点实验室,哈尔滨150080

出　　处：《中国农学通报》2022年第9期115-122,共8页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金面上项目“氮沉降条件下阔叶红松林功能真菌群落结构的响应及其对土壤有机碳动态的调控”(31971527);黑龙江省自然科学基金研究团队项目“寒区农业土壤面源污染微生物调控技术的研究”(TD2019C002)。

摘　　要：AM真菌在没有植物共生的情况下还无法像其他种类真菌一样在培养基上纯培养,这一问题极大地限制了对AM真菌的基础研究和应用研究。为了明确不同丛枝菌根(AM)真菌培养的特点,最终实现AM真菌的纯培养目标,本文归纳并比较了AM真菌与完整植物体共生培养的方法,包括土壤盆栽法、营养液膜法、汽雾栽培法、培养基培养法;总结了AM真菌与植物根系双重培养的方式,包括原生根培养和转化根培养。同时提出了相关后续的研究重点,以期为AM真菌培养及共生机制研究提供参考。In the absence of plant symbiosis, arbuscular mycorrhiza(AM) fungi cannot be purely cultured on medium like other fungi, which greatly restricts the basic and applied research on AM fungi. This study aims at clarifying the development process and characteristics of different culture methods of arbuscular mycorrhiza(AM) fungi, and achieving the goal of pure culture of AM fungi. The authors reviewed and compared the symbiotic culture methods between AM fungi and the whole plant, including pot culture method, nutrient film technique, aeroponics and medium culture. The authors also summarized the dual culture methods between AM fungi and plant roots, including non-transformed root culture and transformed root culture. At last, the authors put forward some future research focuses in order to provide reference for the research of AM fungi culture and symbiotic mechanism.

关 键 词：丛枝菌根真菌 共生培养 双重培养 共生机制 

分 类 号：S154.3[农业科学—土壤学]